Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans par-4 Polyclonal antibody

Uniprot No.

Q9GN62

Target Names

par-4

Alternative Names

par-4 antibody; Y59A8B.14 antibody; Serine/threonine-protein kinase par-4 antibody; EC 2.7.11.1 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans par-4 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Required for cytoplasmic partitioning and asymmetric cell division in early embryogenesis. Controls the asymmetric cell division of the Q.p neuroblast lineage. Involved in mediating cell polarization via regulation of anillin family scaffold proteins. Phosphorylates and restricts the asymmetry effectors mex-5 and mex-6 to the anterior cytoplasm of the zygote and maintains these phosphorylations until fertilization. May phosphorylate par-1. Required for strd-1 localization to the cell cortex of early embryos and may be required for strd-1 protein stabilization. May regulate the integrity of the early embryonic cortex in a strd-1-dependent manner. Phosphorylates and regulates aak-2 in response to oxidative stress and during dauer development. May also play a role in motility, behavioral response, regulation of lifespan and dauer formation through this pathway. Required to establish germline stem cell (GSC) quiescence during dauer development. Acts downstream of unc-40 in dendrite outgrowth. May play a role in cell shedding during embryogenesis, probably by phosphorylating pig-1.

Gene References into Functions

the regulation of the cytoskeleton and cell polarity may contribute significantly to the tumour suppressor function of LKB1/par-4. PMID: 29907081
We report here that din-1S is required in parallel to par-4/LKB1 signaling within the gonad to establish cell cycle quiescence during the onset of the dauer stage. PMID: 27260305
PAR-4/LKB1 impinges on myosin via two pathways, an anillin-dependent pathway that also responds to the cullin CUL-5 and an anillin-independent pathway involving the kinase PIG-1/MELK. PMID: 26416962
Par-4 activates PIG-1 by phosphorylation of T169. PMID: 23267054
the serine-threonine kinase PAR-4 (LKB1) was selectively required for the activity of the UNC-40 pathway in dendrite outgrowth. PMID: 21186357
The Caenorhabditis elegans AMP-activated protein kinase AAK-2 is phosphorylated by LKB1 and is required for resistance to oxidative stress and for normal motility and foraging behavior PMID: 18408008

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Subcellular Location

Cytoplasm, cell cortex.

Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, LKB1 subfamily

Tissue Specificity

Expressed in the gonads, oocytes and early embryos (at protein level).

Database Links

KEGG: cel:CELE_Y59A8B.14

STRING: 6239.Y59A8B.14a

UniGene: Cel.19519

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par-4 Antibody

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