parC Antibody

Code CSB-PA360268XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) parC Polyclonal antibody
Uniprot No.
Target Names
parC
Alternative Names
parC antibody; b3019 antibody; JW2987 antibody; DNA topoisomerase 4 subunit A antibody; EC 5.6.2.2 antibody; Topoisomerase IV subunit A antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) parC protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Topoisomerase IV is essential for chromosome segregation; it is the principal protein responsible for decatenating newly replicated chromosomes. It relaxes supercoiled DNA. MukB stimulates the relaxation activity of topoisomerase IV and also has a modest effect on decatenation.
Gene References into Functions
  1. Among nalidixic acid resistant isolates 13 (28%) showed presence of single nucleotide polymorphism (SNP) in parC (C330-T330). PMID: 27496516
  2. QnrA binds to topoisomerase IV and its subunits in a reaction that does not depend on the presence of DNA, quinolone, or ATP. PMID: 15980397
  3. the centromere-like parC DNA is wrapped around a ParR protein scaffold and works with ParM polymerization to drive active DNA transport during plasmid partition PMID: 17898804
  4. acquisition of a fourth resistance mutation significantly increased fitness especially with the addition of a parC mutation (Topoisomerase IV) to a low-fitness strain carrying resistance mutations in gyrA (DNA Gyrase) and marR (drug efflux regulation). PMID: 19662169
Subcellular Location
Cell membrane; Peripheral membrane protein.
Protein Families
Type II topoisomerase GyrA/ParC subunit family, ParC type 1 subfamily
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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