pasT Antibody

Code CSB-PA927342XA01FQR
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Product Details

Full Product Name
Rabbit anti-Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) pasT Polyclonal antibody
Uniprot No.
Target Names
pasT
Alternative Names
pasT antibody; ratA antibody; yfjG antibody; c3141 antibody; Persistence and stress-resistance toxin PasT antibody; Ribosome association toxin RatA antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Immunogen
Recombinant Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) pasT protein
Immunogen Species
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Toxic component of a type II toxin-antitoxin (TA) system. Binds to 50S ribosomal subunits, preventing them from associating with 30S subunits to form 70S ribosomes. In this strain of E.coli low levels of PasT complement operon disruption, however high levels are toxic; their effects are abrogated by high level expression of cognate antitoxin PasI. Plays a role in persistence after antibiotic exposure and survival of nitrosative stress; the toxic and persistence phenotypes are conferred by the same N-terminal region of the protein, while the stress resistance effects can be uncoupled from them in deletion mutants.
Protein Families
Ribosome association toxin RatA family
Database Links

KEGG: ecc:c3141

STRING: 199310.c3141

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