Patj Antibody

Datasheet

Code	CSB-PA885653XA01DLU
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Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Patj Polyclonal antibody

Uniprot No.

Q9NB04

Target Names

Patj

Alternative Names

Patj antibody; CG12021 antibody; Patj homolog antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Patj protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Involved in cell polarity establishment. Probably participates in the assembly, positioning and maintenance of adherens junctions via its interaction with the SAC complex.

Gene References into Functions

The weakening of adherens junction(AJ) in a PATJ mutant epithelium led first to a loss of Myosin from the AJ, subsequently to a disassembly of the AJ, and finally, to a loss of apical-basal polarity and disruption of the tissue. PMID: 23128243
dPatj possesses an as yet unidentified function that is essential for pupal development. PMID: 22791898
dlt is not required for cell polarity but is needed for cell survival and cell cycle progression. PMID: 14667407
Patj plays a role in retinal morphogenesis and protects against light-dependent degeneration of photoreceptor cells. PMID: 16245332
Has domain-specific early and late functions in regulating the localization and stability of the Crb-Sdt complex in photoreceptor cells. PMID: 16518799

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Subcellular Location

Membrane. Note=Membrane-associated. Localizes to the subapical domain of cells. Sdt is required for its localization. At mitotic cycles 13 and 14, during cellularization, it localizes at the leading edge of the invaginating membranes. It is then exclusively localized to the basal area of columnal epithelial cells. In the germline, it localizes at the membrane of the nurse cells during early and mid oogenesis. Later, it is found in the nuclear membrane in close association with actin filaments that connect the nuclei of the nurse cells with the plasma membrane.

Protein Families

Patj family

Tissue Specificity

Expressed in primary and some secondary epithelial cells such as ectodermal cells, salivary glands, foregut, hindgut and invaginating tracheal cells. Also expressed in specific cells of the peripheral nervous system. Expressed in the germline.

Database Links

KEGG: dme:Dmel_CG12021

STRING: 7227.FBpp0072662

UniGene: Dm.820

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Patj Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background