Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PCAP1 Polyclonal antibody

Uniprot No.

Q96262

Target Names

PCAP1

Alternative Names

PCAP1 antibody; MDP25 antibody; At4g20260 antibody; F1C12.180 antibody; Plasma membrane-associated cation-binding protein 1 antibody; AtPCAP1 antibody; Microtubule-destabilizing protein 25 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PCAP1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

May be involved in intracellular signaling through interaction with PtdInsPs and calmodulin (CaM); may keep PtdInsPs attached to the plasma membrane until Ca(2+)-CaM reaches a competitive concentration subsequent to an increase triggered by a stimulus, thus leading to PtdInsPs release and subsequent activation of InsPs-dependent signaling cascade. Interacts competitively at the N-terminus with calcium ions and CaM (in a calcium-dependent manner), and with the phosphatidylinositol phosphates PtdIns(3,4,5)P(3), PtdIns(3,4)P(2), PtdIns(4,5)P(2) and PtdIns(3,5)P(2). Binds also weakly to PtdIns(3)P, PtdIns(4)P and PtdIns(5)P. Negative regulator of hypocotyl cell elongation by destabilizing cortical microtubules in a calcium-dependent manner. Binds directly to and destabilized microtubules to enhance microtubule depolymerization when cytoplasmic calcium increases. In case of Turnip mosaic virus (TuMV) infection, confers sensitivity by promoting viral cell-to-cell movement through interaction with viral P3N-PIPO.

Gene References into Functions

PCaP1 is involved in the stomatal movement, especially closure process, in leaves and response to excessive copper in root and leaf as a mineral nutrient as a physiological role. PMID: 26979064
MICROTUBULE-DESTABILIZING PROTEIN25 (MDP25) has the actin filament-severing activity of an actin binding protein. PMID: 24424096
MDP25 destabilizes microtubules and exerts its effect on microtubules as a result of transient elevation of cytosolic calcium levels. [MDP25] PMID: 22751329
potyviral P3N-PIPO interacts specifically with the host plasma membrane protein PCaP1 to participate in cell-to-cell movement. PMID: 22511869
MDP25 induces microtubule depolymerization. PMID: 22209764
PCaP1 has a high Cu(2+)-binding capacity with a relatively high affinity. PCaP1 lacks cysteine and histidine residues. A large number of glutamate residues may be involved in the Cu(2+) binding. [PCaP1 cation-binding protein] PMID: 18664522

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Subcellular Location

Cell membrane; Lipid-anchor. Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, plasmodesma. Note=Shuttles from plasma membrane to cytoplasm (e.g. colocalizes with cortical microtubules) upon calcium levels increase. Co-localizes with Turnip mosaic virus (TuMV) P3N-PIPO at the plasmodesmata.

Protein Families

DREPP family

Tissue Specificity

Mostly expressed in the basal region of hypocotyls. Expressed in seedlings, roots, shoots, stems, leaves (e.g. in epidermis and vascular tissues), flowers (e.g. in pistils and anthers) and siliques (at protein level).

Database Links

KEGG: ath:AT4G20260

STRING: 3702.AT4G20260.4

UniGene: At.21638

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PCAP1 Antibody

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