Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PCAP2 Polyclonal antibody

Uniprot No.

Q9LU05

Target Names

PCAP2

Alternative Names

PCAP2 antibody; MAP18 antibody; At5g44610 antibody; K15C23.5 antibody; Plasma membrane-associated cation-binding protein 2 antibody; AtPCAP2 antibody; Lysine rich protein At168 antibody; Microtubule-associated protein 18 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PCAP2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

May be involved in intracellular signaling through interaction with PtdInsPs and calmodulin (CaM); may keep PtdInsPs attached to the plasma membrane until Ca(2+)-CaM reaches a competitive concentration subsequent to an increase triggered by a stimulus, thus leading to PtdInsPs release and subsequent activation of InsPs-dependent signaling cascade (Probable). Binds to microtubules and inhibits tubulin polymerization. Regulates directional cell growth and cortical microtubule organization by destabilizing microtubules (e.g. in cotyledon pavement cells).

Gene References into Functions

Results indicate a function of MICROTUBULE-ASSOCIATED PROTEIN18 (MAP18) in the regulation of Rop GTPase ROP2 (ROP2) activation during root hair growth. PMID: 28314794
maintains nucleus at fixed distance from the apex during root hair growth by modulating actin filaments PMID: 25929794
MAP18 is required for normal pollen tube growth in vitro. PMID: 23463774
The N23 domain of PCaP2 negatively regulates root hair tip growth via processing Ca(2+) and phosphatidylinositol phosphates (PtdInsPs) signals on the plasma membrane, while the residual domain is involved in the polarization of cell expansion. PMID: 23445487
PCaP2 is involved in intracellular signaling through interaction with PtdInsPs and calmodulin in growing root hairs. [PCaP2] PMID: 20061304
MAP18 may play a role in regulating directional cell growth and cortical microtubule organization by destabilizing microtubules.[MAP18] PMID: 17337629

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Subcellular Location

Cell membrane; Lipid-anchor. Cytoplasm, cytoskeleton.

Protein Families

DREPP family

Tissue Specificity

Mostly expressed in the expanding cells, specifically in roots (except in root tips) and flowers (at protein level). Also detected in cotyledons, hypocotyls and trichome stalks.

Database Links

KEGG: ath:AT5G44610

STRING: 3702.AT5G44610.1

UniGene: At.43801

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PCAP2 Antibody

Product Details

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Target Background