Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PCF11 Polyclonal antibody

Uniprot No.

P39081

Target Names

PCF11

Alternative Names

PCF11 antibody; YDR228C antibody; YD9934.13CProtein PCF11 antibody; protein 1 of CF I antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PCF11 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factor NAB4/CFIB and the cleavage and polyadenylation factor (CPF) complex. Independently involved in RNA polymerase II transcript termination. Binds RNA. Seems to bridge RNA polymerase II and the native transcript and may be involved in dismantling the RNA polymerase II elongation complex.

Gene References into Functions

Study describes a novel flower-like structure for the C-terminal CCHC zinc-finger domain of ScPcf11 that is only distantly related to other zinc coordinating domains. PMID: 27780845
Pcf11, a component of the cleavage and polyadenylation complex (CPAC), is also generally required for NRD-dependent transcription termination through the action of its C-terminal domain (CTD)-interacting domain PMID: 25877920
X-ray crystallographic evidence of an extended binding interface of Pcf11p with Clp1p which is likely to be S. cerevisiae specific. PMID: 24508575
Mutations in Pcf11 that perturb Clp1 binding cause temperature-sensitive growth and affect cleavage, polyadenylation and transcription termination. PMID: 21993299
CF IA is reconstituted from Rna14, Rna15, Pcf11, and Clp1 proteins in 2:2:1:1 stoichiometry. PMID: 22026644
Pcf11 CID recognizes the trans-trans form of the phosphorylated peptide PMID: 15665873
NMR backbone assignment of PCF11 CTD binding domain [letter] PMID: 15929005
Molecular model of a ternary complex of Clp1-ATP and the Clp1-binding region of Pcf11. PMID: 17151076
Tethering of Pcf11 to nascent mRNA is sufficient to enhance Yra1 recruitment. Interaction with Pcf11 can therefore explain Yra1 binding to the TEC independently of Sub2 PMID: 19110458

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Subcellular Location

Nucleus.

Database Links

KEGG: sce:YDR228C

STRING: 4932.YDR228C

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PCF11 Antibody

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