LUX Antibody

Code CSB-PA577791XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LUX Polyclonal antibody
Uniprot No.
Target Names
LUX
Alternative Names
LUX antibody; PCL1 antibody; At3g46640 antibody; F12A12.160 antibody; Transcription factor LUX antibody; Protein LUX ARRHYTHMO antibody; Protein PHYTOCLOCK 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana LUX protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor that is essential for the generation of the circadian clock oscillation. Is necessary for activation of CCA1 and LHY expression. Is coregulated with TOC1 and seems to be repressed by CCA1 and LHY by direct binding of these proteins to the evening element in the LUX promoter. Directly regulates the expression of PRR9, a major component of the morning transcriptional feedback circuit, by binding specific sites on PRR9 promoter. Binds to its own promoter, inducing a negative auto-regulatory feedback loop within the core clock. Binds to ELF3 and associates with ELF4 in a diurnal complex which is required for the expression of the growth-promoting transcription factors PIF4 and PIF5 and subsequent hypocotyl growth in the early evening.
Gene References into Functions
  1. Transcriptional regulation of LUX by CBF1 mediates cold input to the circadian clock in Arabidopsis. PMID: 24954045
  2. The in vitro Dna-binding specificity of LUX, its role in the expression of PRR9, and its feedback properties are reported. PMID: 21236673
  3. LUX ARRHYTHMO (LUX) encodes a Myb domain protein essential for circadian rhythms. [LUX] PMID: 16006522
  4. The PCL1 gene is the clock oscillator gene essential to the generation of clock oscillation in the higher plant. [PCL1] PMID: 16164597
Subcellular Location
Nucleus.
Database Links

KEGG: ath:AT3G46640

STRING: 3702.AT3G46640.3

UniGene: At.47951

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