PCS1 Antibody

Code CSB-PA831442XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PCS1 Polyclonal antibody
Uniprot No.
Target Names
PCS1
Alternative Names
PCS1 antibody; EMB24 antibody; At5g02190 antibody; T7H20.240 antibody; Aspartic proteinase PCS1 antibody; EC 3.4.23.- antibody; Aspartic protease 38 antibody; AtASP38 antibody; Protein EMBRYO DEFECTIVE 24 antibody; Protein PROMOTION OF CELL SURVIVAL 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PCS1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Embryo-specific aspartic protease that limits programmed cell death during reproductive development. Possesses peptidase activity toward casein in vitro.
Gene References into Functions
  1. C-terminal parts involved in arsenite- [As(III)] dependent activation of AtPCS1, the primary Arabidopsis phytochelatin synthase, were identified. PMID: 29281059
  2. The heterologous expression of AtPCS1 in tobacco plants exposed to arsenic plus cadmium enhances phytochelatin levels, root As/Cd accumulation and pollutants detoxification, but does not prevent root cyto-histological damages. PMID: 26563149
  3. Cadmium and phytochelatin levels were determined in protoplasts and vacuoles isolated from leaves of Nicotiana tabacum expressing either of two phytochelatin synthase genes, AtPCS1 and CePCS. PMID: 20381898
  4. contributes specifically to the cytosolic turnover of glutathione conjugates PMID: 20304971
  5. Phytochelatin synthase is regulated by protein phosphorylation at a threonine residue near its catalytic site. PMID: 19653625
  6. PROMOTION OF CELL SURVIVAL 1 (PCS1) gene in Arabidopsis, which encodes an aspartic protease, has an important role in determining the fate of cells in embryonic development and in reproduction processes [PCS1] PMID: 15723040
  7. results enable us to propose an advanced model of PC synthesis, describing substrate specificity, heavy metal requirement, and the active site in the PCS1 enzyme PMID: 15809863

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Subcellular Location
Endoplasmic reticulum.
Protein Families
Peptidase A1 family
Tissue Specificity
Expressed specifically in developing gametophytes and developing seeds.
Database Links

KEGG: ath:AT5G02190

STRING: 3702.AT5G02190.1

UniGene: At.4814

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