nub Antibody

Code CSB-PA329066XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) nub Polyclonal antibody
Uniprot No.
Target Names
nub
Alternative Names
nub antibody; OCT1 antibody; pdm-1 antibody; POU-19 antibody; TWN antibody; CG34395 antibody; Protein nubbin antibody; POU domain protein 1 antibody; PDM-1 antibody; Protein twain antibody; dOCT1 antibody; dPOU-19 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) nub protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
DNA-binding regulatory protein implicated in early development. Involved in neuronal cell fate decision. Repressed directly or indirectly by the BX-C homeotic proteins.
Gene References into Functions
  1. Overexpression of Nub-PB enhanced bacterial clearance of ingested Erwinia carotovora carotovora 15 PMID: 29499056
  2. this study shows that Pdm1 is necessary for a beneficial gut microbiota and normal lifespan PMID: 27231014
  3. We suggest that Nub protein plays an ancient role, shared with mammalian Oct/POU transcription factors, to moderate responses to immune challenge, thereby increasing the tolerance to biotic stress. PMID: 24010524
  4. Pdm-1 and Drosophila octamer elements dictate the coordinated transcription of core histone genes PMID: 20097756
  5. maintaining high levels of Mitimere or Nubbin, two POU proteins, in a ganglion mother cell causes it to undergo self-renewing asymmetric divisions PMID: 14973280
  6. Pdm and Cas specify late-born neuronal identity; that Pdm and Cas act combinatorially to specify a temporal identity distinct from either protein alone, and that Cas repression of pdm expression regulates the generation of neuronal diversity. PMID: 16980589
  7. Nub and Tsh domains share some of the attributes of classical compartments, but lack their stringent and immobile boundaries. PMID: 17313943
  8. These results provide a new neuroblast lineage for investigating temporal identity and reveal the importance of Pdm and Cas as timing factors that close temporal identity windows. PMID: 18832394

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Subcellular Location
Nucleus.
Protein Families
POU transcription factor family, Class-2 subfamily
Tissue Specificity
Initial expression in cellular blastoderm stage, then in ectodermal stripes during germband extension. Broad expression in the neuroectoderm followed by limitation to discrete subsets of CNS cells, and expression in specific PNS neurons and support cells.
Database Links

KEGG: dme:Dmel_CG34395

STRING: 7227.FBpp0111554

UniGene: Dm.4744

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