pept-1 Antibody

Code CSB-PA633658XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans pept-1 Polyclonal antibody
Uniprot No.
Target Names
pept-1
Alternative Names
pept-1 antibody; cptb antibody; opt-2 antibody; pep-2 antibody; K04E7.2 antibody; Peptide transporter family 1 antibody; Di-/tri-peptide transporter CPTB antibody; Oligopeptide transporter 1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans pept-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Low-affinity peptide transporter that is necessary for proton-dependent uptake of di- or tripeptides, and to a minor extent tetrapeptides, in the intestine. Transport is independent of sodium and chloride ions. Controls the uptake of dietary fatty acids, plays a role in fatty acid synthesis and is responsible for dipeptide-induced acidification of the intestine. Regulates cellular pH differences together with the antiporter protein, nhx-2. Amino acid uptake and absorption levels influence the insulin signaling/daf-2 and let-363/TOR pathways, subsequently affecting the stress response and longevity of the organism. It is required for the uptake of the L-enantiomers of various amino acids, including L-glutamate. In response to the availability of amino acid nutrients, may play a role in promoting reproduction and fertility.
Gene References into Functions
  1. intracellular peptide hydrolysis and amino acid concentration are a part of a sensing system that controls PEPT-1 expression and function and that involves the TOR complexes TORC1 and TORC2 [PEPT-1][TORC1][TORC2] PMID: 21980510
  2. Intestinal amino acid availability via PEPT-1 affects TORC1/2 signaling and the unfolded protein response. PMID: 24999909
  3. Loss of PEPT-1 decreases intestinal proton influx leading to a higher uptake of free fatty acids with fat accumulation PMID: 19621081
  4. pep-2 cross-talks with both the C. elegans target of rapamycin (TOR) and the DAF-2/insulin-signaling pathways PMID: 15155758
Subcellular Location
Apical cell membrane; Multi-pass membrane protein. Note=Colocalizes with nhx-2 along the apical membrane of the intestinal cells.
Protein Families
PTR2/POT transporter (TC 2.A.17) family
Tissue Specificity
Expressed specifically in the intestine.
Database Links

KEGG: cel:CELE_K04E7.2

STRING: 6239.K04E7.2.1

UniGene: Cel.19638

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