Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans lin-42 Polyclonal antibody

Uniprot No.

Q65ZG8

Target Names

lin-42

Alternative Names

lin-42 antibody; F47F6.1 antibody; Period protein homolog lin-42 antibody; Abnormal cell lineage protein 42 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans lin-42 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcriptional repressor which interacts with the promoter region of target genes. Has a specific role in developmental timing where it regulates temporal expression of a number of miRNAs and mRNAs. Controls temporal cell fate transition during embryonic and early larval development by restricting the expression of specific miRNAs, including let-7, miR-48, lin-4, miR-35 and miR-58. Restricts the accumulation of lin-29 in the hypodermis to the larval L4 stage, thus controlling terminal differentiation of seam cells. Has a role in the miRNA-mediated specification of asymmetric gene expression patterns in gustatory neurons. May also regulate genes involved in other biological processes including transport, small molecule metabolism, and growth. Inhibits dauer formation, by antagonizing daf-12.; Specifically required for maintaining the timing of larval development and molting cycle rhythms.

Gene References into Functions

The method was validated with a lin-42 mutant strain that shows delayed development relative to wild-type animals and with a daf-2 mutant that shows an extended second larval stage PMID: 26294666
A key function of lin-42 in developmental timing is to dampen pri-miRNAs levels, preventing their premature expression as mature miRNAs. PMID: 25319259
lin-42 functions to negatively regulate the transcriptional output of multiple miRNAs and mRNAs and therefore coordinates the expression levels of genes that dictate temporal cell fate PMID: 25032706
The Period protein homolog LIN-42 negatively regulates microRNA biogenesis in C. elegans. PMID: 24699545
Inactivation of the heterochronic gene lin-42a leads to arrhythmic molts and abnormal epidermal stem cell dynamics. Oscillatory expression in the epidermis peaks during the molts; forced expression leads to anachronistic larval molts and adult lethargy. PMID: 22137474
The C. elegans developmental timing protein LIN-42 regulates diapause in response to environmental cues. PMID: 20843862
LIN-42 has a dynamic expression pattern; its levels oscillate relative to the molts during postembryonic development. PMID: 16300753

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Subcellular Location

Nucleus. Cytoplasm.

Database Links

STRING: 6239.F47F6.1b

UniGene: Cel.18253

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lin-42 Antibody

Product Details

Customer Reviews and Q&A

Target Background