Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) PGRP-LE Polyclonal antibody

Uniprot No.

Q9VXN9

Target Names

PGRP-LE

Alternative Names

PGRP-LE antibody; CG8995 antibody; Peptidoglycan-recognition protein LE antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) PGRP-LE protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Peptidoglycan-recognition protein that plays a key role in innate immunity by binding to murein peptidoglycans (PGN) of Gram-negative bacteria and activating the imd/Relish pathway. Has no activity against on Gram-positive bacteria. Binds to diaminopimelic acid-type PGN (DAP-type PGN), an activator of the imd/Relish pathway. Functions synergistically with PGRP-LC in producing resistance to E.coli and B.megaterium infections, which have the DAP-type peptidoglycan. Acts both upstream and in parallel with PGRP-LC in the imd/Relish pathway, and is required for infection-dependent activation of melanization. Required for Relish processing and nuclear translocation following proteolytic cleavage. Its localization suggests a role in the recognition and subsequent activation of the signaling at the first point of contact with invading bacteria.

Gene References into Functions

Protofibrils of peptidoglycan receptors PGRP-LC and PGRP-LE seed immune deficiency protein (Imd) amyloid polymerization via Imd RIP homotypic interaction motif (RHIM), and the formation of functional Imd amyloid fibril is required for NF-kappaB signaling. PMID: 29045898
PGRP-LE functions as a gut bacterial sensor that induces balanced responses to infectious bacteria and tolerance to microbiota. PMID: 22901536
our study delineates how a combination of extracellular sensing by PGRP-LC isoforms and intracellular sensing through PGRP-LE provides sophisticated mechanisms to detect and differentiate between infections in Drosophila. PMID: 22772451
PGRP-LE and PGRP-LC play a crucial role in detecting extracellular and intracellular bacteria with DAP-type peptidoglycans to induce various innate immune responses PMID: 20089584
PGRP-LE acts as a pattern-recognition receptor to the diaminopimelic acid-type peptidoglycan and activates both the proteolytic cascade and intracellular signaling in immunity. PMID: 12359879
PGRP-LE acts both upstream and in parallel with PGRP-LC in the imd pathway, and is required for infection-dependent activation of melanization in Drosophila. PMID: 15538387
Full-length PGRP-LE acts as an intracellular receptor for monomeric peptidoglycan. A form of PGRP-LE with only the PGRP domain functions extracellularly to enhance PGRP-LC-mediated peptidoglycan recognition on the cell surface. PMID: 16767093
The function of PGRP-LE in innate immunity as a recognition receptor for intracellular bacteria was studied. PMID: 18769162

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Subcellular Location

Secreted. Note=However, no signal sequence are predicted by sequence analysis tools.

Protein Families

N-acetylmuramoyl-L-alanine amidase 2 family

Tissue Specificity

Expressed in hemolymph. Localizes at the lumenal surface of the trachea (at protein level).

Database Links

KEGG: dme:Dmel_CG8995

STRING: 7227.FBpp0300347

UniGene: Dm.3371

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PGRP-LE Antibody

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