PHO81 Antibody

Code CSB-PA323513XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PHO81 Polyclonal antibody
Uniprot No.
Target Names
PHO81
Alternative Names
PHO81 antibody; YGR233C antibody; G8567 antibody; Phosphate system positive regulatory protein PHO81 antibody; CDK inhibitor PHO81 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PHO81 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Inhibits the kinase activity of the cyclin-CDKs PHO80-PHO85 and PCL7-PHO85 under low-phosphate conditions.
Gene References into Functions
  1. Full-length Pho81 is required to control trehalose metabolism and the PDS targets in Saccharomyces. PMID: 15926040
  2. myo-d-inositol heptakisphosphate is necessary for Pho81-dependent inhibition of Pho80-Pho85 in vitro; fndings reveal regulation of a cyclin-CDK complex by a metabolite & suggest that a complex metabolic network mediates signaling of phosphate availability PMID: 17412959
Subcellular Location
Cytoplasm. Nucleus. Note=Localizes predominantly to the nucleus in both high- and low-phosphate conditions.
Database Links

KEGG: sce:YGR233C

STRING: 4932.YGR233C

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