phoP Antibody

Code CSB-PA921625XA01SXB
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Product Details

Full Product Name
Rabbit anti-Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) phoP Polyclonal antibody
Uniprot No.
Target Names
phoP
Alternative Names
phoP antibody; STM1231 antibody; Virulence transcriptional regulatory protein PhoP antibody; Acid shock protein 29 antibody; ASP29 antibody
Raised in
Rabbit
Species Reactivity
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Immunogen
Recombinant Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) phoP protein
Immunogen Species
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Member of the two-component regulatory system PhoP/PhoQ which regulates the expression of genes involved in virulence, adaptation to acidic and low Mg(2+) environments and resistance to host defense antimicrobial peptides. Essential for intramacrophage survival of S.typhimurium. In low periplasmic Mg(2+), PhoQ phosphorylates PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg(2+), PhoQ dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG. Essential for transcription of spiC inside macrophages by controlling the expression of the two-component regulatory system SsrB/SpiR (SsrA) and Pir at transcriptional and post-transcriptional levels respectively. Promotes expression of the two-component regulatory system PmrA/PmrB via activation of pmrD gene. Is required to attenuate bacterial growth within fibroblast cells and to enhance bacterial resistance to bile in intestinal cells. Negatively regulates prgH, which is required for invasion of epithelial cells. PhoP uses multiple mechanisms to promote transcription and activates promoters for PAG at low (uM range) Mg(2+) concentrations (Probable). Involved in acid tolerance.
Gene References into Functions
  1. Second monomer binding is the rate-limiting step in the formation of the dimeric PhoP-DNA complex PMID: 22268791
  2. proteomic analysis of the PhoP regulon in Salmonella enterica serovar Typhi versus Typhimurium PMID: 19746165
  3. the promoter binding ability of the PhoP and PmrA proteins occurring in low Mg2+ is correlated with phosphorylation of these proteins in vivo PMID: 15569664
  4. analysis of the spatial-temporal patterns of in vivo expression of genes of the PhoP and PmrA regulons and identification of the in vivo signals modulating their transcription PMID: 16237024
  5. the response regulator PhoP was found to play a key role in the repression of tlpA in conjunction with two other regulators, RpoS and TlpA itself PMID: 16782389
  6. Here we show that the srfABC operon is negatively regulated by RcsB and by PhoP. PMID: 17616593
  7. in PhoP-constitutive strains the outer membrane serves as a very effective permeability barrier, whereas in phoP null mutants the outer membrane is severely compromised in its barrier function PMID: 17693506
  8. This paper shows that mutations in hilA, hilC, or hilD reduce expression of siiE, and mutations in hilE or phoP enhance siiE expression. PMID: 18160484
  9. The authors demonstrate that in PhoP/PhoQ-inducing environments PhoP displays preferential localization to one cell pole, while being homogeneously distributed in the bacterial cytoplasm in repressing conditions. PMID: 18761685

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Subcellular Location
Cytoplasm.
Database Links

KEGG: stm:STM1231

STRING: 99287.STM1231

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