ph-p Antibody

Code CSB-PA331073XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) ph-p Polyclonal antibody
Uniprot No.
Target Names
ph-p
Alternative Names
ph-p antibody; CG18412 antibody; Polyhomeotic-proximal chromatin protein antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) ph-p protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. Component of the PcG multiprotein PRC1 complex, a complex that acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-118', rendering chromatin heritably changed in its expressibility. Plays a role in regulating the expression of other pair-rule genes such as eve, ftz, and H.
Gene References into Functions
  1. Organization of PcG proteins into small, abundant clusters on chromatin through Polyhomeotic protein sterile alpha motif polymerization activity may shape genome architecture through chromatin interactions. PMID: 26759081
  2. Polycomb protein Polyhomeotic mutants lacking the S/T stretch reproduce the phenotype of ogt mutants PMID: 25468754
  3. Ph sterile alpha motif polymerization is required for Ph-mediated gene repression. PMID: 22275371
  4. Data show that cells in an epithelium expressing different levels of polyhomeotic sort out indicating differential adhesive properties between the cell populations. PMID: 21085656
  5. We analyzed the cell cycle role of the proximal subunit of Polyhomeotic (PhP) in Drosophila PMID: 20045683
  6. Our results show a functional relationship between the PcG gene expression and the JAK/STAT pathway, both of which are found to be perturbed in tumorigenesis. PMID: 19923887
  7. Ph SAM domain forms a helical polymer structure, providing a likely mechanism for the extension of PcG complexes PMID: 11992127
  8. neofunctionalization has been achieved in the Drosophila ph genes through the action of strong positive selection and the inactivation of gene conversion in part of the gene. PMID: 18381818

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Subcellular Location
Nucleus.
Tissue Specificity
Salivary glands.
Database Links

KEGG: dme:Dmel_CG18412

STRING: 7227.FBpp0070416

UniGene: Dm.5733

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