Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PHR1 Polyclonal antibody

Uniprot No.

Q94CL7

Target Names

PHR1

Alternative Names

PHR1 antibody; At4g28610 antibody; T5F17.60 antibody; Protein PHOSPHATE STARVATION RESPONSE 1 antibody; AtPHR1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PHR1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcription factor involved in phosphate starvation signaling. Binds as a dimer to P1BS, an imperfect palindromic sequence 5'-GNATATNC-3', to promote the expression of inorganic phosphate (Pi) starvation-responsive genes. SPX1 is a competitive inhibitor of this DNA-binding. PHR1 binding to its targets is low Pi-dependent. Regulates the expression of miR399. Regulates the expression of IPS1 (At3g09922), a non-coding RNA that mimics the target of miR399 to block the cleavage of PHO2 under Pi-deficient conditions. Regulates lipid remodeling and triacylglycerol accumulation during phosphorus starvation. Required for the shoot-specific hypoxic response. Regulates FER1 expression upon phosphate starvation, linking iron and phosphate homeostasis. Contributes to the homeostasis of both sulfate and phosphate in plants under phosphate deficiency. Required for adaptation to high light and retaining functional photosynthesis during phosphate starvation. Involved in the coregulation of Zn and Pi homeostasis.

Gene References into Functions

light and ethylene coordinately regulate PHR1 expression and Phosphate starvation responses through signaling convergence at the PHR1 promoter. PMID: 28842534
These data suggest that, in the Pi-restored condition, AtSPX1 can bind to monomeric AtPHR1 in solution and therefore regulate PSI gene expression by tuning the AtPHR1-DNA-binding equilibrium. PMID: 28887383
The relative strength of the SPX1/PHR1 interaction is thus directly influenced by phosphate (Pi), providing a link between Pi perception and signaling. PMID: 25271326
that both PHR1 and PHL1 are major factors involved in the regulation of iron homeostasis PMID: 23788639
PHR1 plays an important role in sulfate inter-organ transport. PMID: 21261953
PHR1 and PHL1 are partially redundant transcription factors acting as central integrators of phosphate starvation responses. PMID: 20838596

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Subcellular Location

Nucleus.

Protein Families

MYB-CC family

Database Links

KEGG: ath:AT4G28610

STRING: 3702.AT4G28610.1

UniGene: At.4525

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PHR1 Antibody

Product Details

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Target Background