PHT1-1 Antibody

Datasheet

Code	CSB-PA837777XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PHT1-1 Polyclonal antibody

Uniprot No.

Q8VYM2

Target Names

PHT1-1

Alternative Names

PHT1-1 antibody; APT2 antibody; PHT1 antibody; PT1 antibody; At5g43350 antibody; MWF20.4 antibody; Inorganic phosphate transporter 1-1 antibody; AtPht1;1 antibody; H(+)/Pi cotransporter antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PHT1-1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

High-affinity transporter for external inorganic phosphate. Acts as a H(+):phosphate symporter in both low- and high-Pi conditions. Confers sensitivity to arsenate.

Gene References into Functions

The phosphate-binding site residues were shown to be well conserved between all the plant PHT1 proteins and Saccharomyces cerevisiae PHO84. PMID: 27911737
ALIX mediates high-affinity phosphate transporter PHT1.1 trafficking to maintain phosphate homeostasis. PMID: 26342016
A model for differential trafficking of PHT1 to the plasma membrane or vacuole as a function of phosphate concentration, is proposed. PMID: 21521698
An Arsenic(V)-tolerant mutant of Arabidopsis thaliana named pht1;1-3, was identified which harbors a semidominant allele coding for the high-affinity Pi transporter PHT1;1. pht1;1-3 displays a slow rate of As(V) uptake. PMID: 17400898

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Major facilitator superfamily, phosphate:H(+) symporter (TC 2.A.1.9) family

Tissue Specificity

Mostly expressed in roots, especially in trichoblasts and in emerging secondary roots and root hairs, but not in root tips. Also present in hydathodes, axillary buds and peripheral endosperm of germinating seeds.

Database Links

KEGG: ath:AT5G43350

STRING: 3702.AT5G43350.1

UniGene: At.49125

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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PHT1-1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background