PIA2 Antibody

Datasheet

Code	CSB-PA150505XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PIA2 Polyclonal antibody

Uniprot No.

Q9FNP4

Target Names

PIA2

Alternative Names

PIA2 antibody; ANK6 antibody; At5g61230 antibody; MAF19.22 antibody; Phytochrome-interacting ankyrin-repeat protein 2 [Cleaved into: Protein ANKYRIN REPEAT 6 antibody; mitochondrial] antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PIA2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Promotes anthocyanin accumulation through interaction with PHYA, especially in response to far-red light, high light and sucrose treatment, probably by triggering A3G2XYLT/UF3GT expression. Required for gametophytes development as well as male-female gamete recognition during fertilization, possibly by regulating mitochondrial gene expression. Represses PHYA-mediated PIF3 phosphorylation.

Gene References into Functions

These results suggest that PIA2 modulates phyA-mediated PIF3 phosphorylation by physical interaction with PIF3 and that the secondary structure of the PIA2 N-terminus is important in this modulation. PMID: 27143545
PIA2 interacts with and is phosphorylated by phytochrome A. PIA2 functions in the regulation of anthocyanin biosynthesis in Arabidopsis. [PIA2] PMID: 21395597
ANK6 plays a central role in male-female gamete recognition, possibly by regulating mitochondrial gene expression. PMID: 21123745

Subcellular Location

[Phytochrome-interacting ankyrin-repeat protein 2]: Cytoplasm. Nucleus.; [Protein ANKYRIN REPEAT 6, mitochondrial]: Mitochondrion.

Tissue Specificity

Mostly expressed in flowers, cotyledons, leaves and siliques, and, to a lower extent, in roots and stems. Also detected at low levels in seedlings grown in continuous dark or light conditions. Expressed in male and female gametophytes.

Database Links

KEGG: ath:AT5G61230

STRING: 3702.AT5G61230.1

UniGene: At.22973

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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