PIE1 Antibody

Datasheet

Code	CSB-PA187810XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PIE1 Polyclonal antibody

Uniprot No.

Q7X9V2

Target Names

PIE1

Alternative Names

PIE1 antibody; CHR13 antibody; At3g12810 antibody; MBK21.19 antibody; Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 antibody; EC 3.6.4.12 antibody; Independent early flowering 1 protein antibody; Protein CHROMATIN REMODELING 13 antibody; AtCHR13 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PIE1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant H2A.F/Z leading to transcriptional regulation of selected genes (e.g. FLC) by chromatin remodeling. Probable DNA-dependent ATPase. Not involved in the repression of FLC in gametophytes, but required for the reactivation of FLC in early embryos and for the maintenance of full activation of FLC in late embryos.

Gene References into Functions

Data indicate that mutations in genes for SWR1 complex subunits photoperiod-independent Early Flowering1 (PIE1), actin-related protein6 (ARP6), and SWR1 complex6 (SWC6) cause hypersensitivity to various DNA damaging agents. PMID: 23780875
The identification and characterization of SERRATED LEAVES AND EARLY FLOWERING (SEF) in A. thaliana and its interactions with other proteins, including ATARP6 and PIE1, are reported. PMID: 17142478
Arabidopsis SWC6 (AtSWC6), SUPPRESSOR OF FRIGIDA 3 (SUF3) and PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (PIE1) are homologs of SWC6, ARP6 and SWR1 with roles in development, including floral repression through full activation of FLOWERING LOCUS C PMID: 17470967

Subcellular Location

Nucleus.

Protein Families

SNF2/RAD54 helicase family, SWR1 subfamily

Tissue Specificity

Expressed in ovules, but not in stamens.

Database Links

KEGG: ath:AT3G12810

STRING: 3702.AT3G12810.1

UniGene: At.39549

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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