Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) pfh1 Polyclonal antibody

Uniprot No.

Q9UUA2

Target Names

pfh1

Alternative Names

pfh1 antibody; pif1 antibody; rph1 antibody; SPBC887.14c antibody; ATP-dependent DNA helicase pfh1 antibody; EC 3.6.4.12 antibody; DNA repair and recombination helicase pfh1 antibody; PIF1 helicase homolog antibody; RRM3/PIF1 homolog 1 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) pfh1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Involved in the maintenance of mitochondrial (mtDNA). Required for both repair of mitochondrial DNA and recognition of a recombinogenic signal characterized by a 26-bp palindromic at sequence in the ery region of mitochondrial DNA. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. Required for the completion of S-phase.

Gene References into Functions

The nuclear isoform of Pfh1 (nPfh1) unwound both types of structure, and although the G4-stabilizing compound Phen-DC3 significantly enhanced their stability, nPfh1 still resolved them efficiently PMID: 27185885
In budding and fission yeasts, Pif1 family helicases impact both telomerase-mediated and semi-conservative replication of telomeric DNA as well as recombination-mediated telomere lengthening. (Review) PMID: 27233114
Pfh1 is an accessory DNA helicase that interacts with the replisome and promotes replication and suppresses DNA damage at hard-to-replicate sites. PMID: 27611590
Pfh1 binds telomeric DNA.Pfh1 facilitates telomere replication.Pfh1 is a positive regulator of telomere length. PMID: 25303777
The proteome of cells devoid of Pfh1 were analyzed and determined that gene products up- and down-regulated upon iron depletion in wild-type cells are constitutively misregulated in this mutant. PMID: 23115244
Pfh1 promotes DNA replication and separation of converged replication forks and suppresses DNA damage at hard-to-replicate sites. PMID: 22426534
These data demonstrate that Pfh1p has essential roles in the replication of both nuclear and mitochondrial DNA. PMID: 18725402

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Subcellular Location

[Isoform Nuclear]: Nucleus, nucleolus.; [Isoform Mitochondrial]: Mitochondrion.

Protein Families

Helicase family, PIF1 subfamily

Database Links

KEGG: spo:SPBC887.14c

STRING: 4896.SPBC887.14c.1

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pfh1 Antibody

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