PIF3 Antibody

Code CSB-PA524969XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PIF3 Polyclonal antibody
Uniprot No.
Target Names
PIF3
Alternative Names
PIF3 antibody; BHLH8 antibody; EN100 antibody; PAP3 antibody; At1g09530 antibody; F14J9.19 antibody; Transcription factor PIF3 antibody; Basic helix-loop-helix protein 8 antibody; AtbHLH8 antibody; bHLH 8 antibody; Phytochrome-associated protein 3 antibody; Phytochrome-interacting factor 3 antibody; Transcription factor EN 100 antibody; bHLH transcription factor bHLH008 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PIF3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor acting in the phytochrome signaling pathway. Activates transcription by binding to the G box (5'-CACGTG-3'). Acts as a negative regulator of phytochrome B signaling. Represses chlorophyll biosynthesis and photosynthesis in the dark. Recruits the histone deacetylase HDA15 to the promoters of chlorophyll biosynthetic and photosynthetic genes. HDA15 represses their transcription by histone deacetylation.
Gene References into Functions
  1. PIF degradation by DELLAs is required to coordinate light and gibberellins signals. PMID: 27282989
  2. study uncovers an important role of PIF3 in Arabidopsis freezing tolerance by negatively regulating the expression of genes in the CBF pathway PMID: 28739888
  3. PIF3 and TOC1 display coincident cobinding to dawn-phased genes under diurnal short-day conditions. They interact and colocalize in the nucleus. TOC1 acts directly as a transcriptional repressor of PIF3, which itself acts intrinsically as a transcriptional activator so that PIF3 and TOC1 act antagonistically in regulating the expression of their cotarget genes. PMID: 27071129
  4. Under negative day-night temperature difference, lower auxin biosynthesis activity limits the signaling in this pathway, resulting in low activity of PIF3 and short hypocotyls. PMID: 25516603
  5. PIF3 phosphorylation induces, and is necessary for, recruitment of LRB [Light-Response Bric-a-Brack/Tramtrack/Broad (BTB)] E3 ubiquitin ligases to the PIF3-phyB complex. PMID: 24904166
  6. Data indicate that HDA15 and PIF3 cotarget to the genes involved in chlorophyll biosynthesis and photosynthesis in the dark and repress gene expression. PMID: 23548744
  7. These results showed an altered response to light in seedlings with an impaired PIF3/MIDA regulatory network. PMID: 22499182
  8. directly activated by ETHYLENE-INSENSITIVE 3 and indispensible for ethylene-induced hypocotyl elongation in light PMID: 22818915
  9. The PIF3 protein abundance oscillates under diurnal conditions as a result of a progressive decline in PIF3 protein degradation mediated by photoactivated phyB, and consequent accumulation of the bHLH factor during the dark period. PMID: 22409654
  10. PIF3 regulates genes misexpressed in the dark, named MIDA genes PMID: 22108407
  11. PIF3 acts transiently, and its major function is to mediate phytochrome-induced signaling during the developmental switch from skotomorphogenesis to photomorphogenesis and/or dark to light transitions PMID: 15155879
  12. PIF3 may function in early phytochrome signaling at the dark-to-light transition, not only during initial seedling deetiolation, but daily at dawn under diurnal light-dark cycles. PMID: 15505214
  13. Data suggest that PIF3 may represent the primary intermolecular signaling transaction of the activated photoreceptor, tagging the target protein for proteosomal degradation. PMID: 16885032
  14. Data provide evidence that the mechanism by which PIFs3operate on the phyB signaling pathway under prolonged red light is through maintaining low phyB protein levels, in an additive or synergistic manner. PMID: 18252845
  15. Data show that dephosphorylation of PIF3 is mediated by phytochrome-associated protein phosphatase type 2C. PMID: 18564962
  16. PIF3 was circadian regulated in dark-grown seedlings. PMID: 19380736

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Subcellular Location
Nucleus.
Database Links

KEGG: ath:AT1G09530

STRING: 3702.AT1G09530.1

UniGene: At.10926

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