PIF5 Antibody

Code CSB-PA772821XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PIF5 Polyclonal antibody
Uniprot No.
Target Names
PIF5
Alternative Names
PIF5 antibody; BHLH65 antibody; EN103 antibody; PIL6 antibody; At3g59060 antibody; F17J16.110 antibody; Transcription factor PIF5 antibody; Basic helix-loop-helix protein 65 antibody; AtbHLH65 antibody; bHLH 65 antibody; Phytochrome interacting factor-like 6 antibody; Phytochrome-interacting factor 5 antibody; Transcription factor EN 103 antibody; bHLH transcription factor bHLH065 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PIF5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor acting negatively in the phytochrome B signaling pathway to promote the shade-avoidance response. Regulates PHYB abundance at the post-transcriptional level, possibly via the ubiquitin-proteasome pathway. Promotes ethylene activity in the dark. May regulate the expression of a subset of genes by binding to the G-box motif. Might be involved in the integration of light-signals to control both circadian and photomorphogenic processes. Activated by CRY1 and CRY2 in response to low blue light (LBL) by direct binding at chromatin on E-box variant 5'-CA[CT]GTG-3' to stimulate specific gene expression to adapt global physiology (e.g. hypocotyl elongation in low blue light).
Gene References into Functions
  1. PIF5 is a key factor that positively regulates dark-induced senescence upstream of ORE1 and regulates chlorophyll breakdown upstream of SGR and NYC1. PMID: 26089152
  2. Phytochrome interacting factors 4 and 5 are part of an inhibitory mechanism that represses the expression of some light-responsive genes in the dark and they are needed for full expression of several growth-related genes in the light. [PIF5] PMID: 19619162
  3. Data indicate that overexpressed PIF5 causes altered ethylene levels, which promote the triple response in darkness, whereas in the light, the interaction of photoactivated phyB with PIF5 causes degradation of the photoreceptor protein. PMID: 18065691
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in roots, leaves, stems, and flowers.
Database Links

KEGG: ath:AT3G59060

STRING: 3702.AT3G59060.2

UniGene: At.43437

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