PIK1 Antibody

Code CSB-PA327934XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PIK1 Polyclonal antibody
Uniprot No.
Target Names
PIK1
Alternative Names
PIK1 antibody; YNL267W antibody; N0795 antibody; Phosphatidylinositol 4-kinase PIK1 antibody; PI4-kinase antibody; PtdIns-4-kinase antibody; EC 2.7.1.67 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PIK1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol 1,4,5,-trisphosphate. PIK1 is part of a nuclear phosphoinositide cycle and could control cytokinesis through the actin cytoskeleton.
Gene References into Functions
  1. Inhibition of Gga2-Pik1 binding resulted in reduced Pik1 localization and delayed accumulation of phosphatidylinositol 4-phosphate to the trans-Golgi network. PMID: 28289207
  2. Thus, our results suggest that pik1(+) is required for the maintenance of circular chromosomes. PMID: 29410177
  3. Data show that Gga2p binds directly to the Gga2p binds directly to the trans-Golgi network (TGN) PtdIns(4)-kinase Pik1p and contributes to Pik1p recruitment. PMID: 22344030
  4. Catalytically inactive derivatives of these compartment-restricted Pik1 constructs indicated that PtdIns4P must be generated both in the nucleus and at the Golgi for normal cell function. PMID: 16365163
Subcellular Location
Nucleus.
Protein Families
PI3/PI4-kinase family, Type III PI4K subfamily
Database Links

KEGG: sce:YNL267W

STRING: 4932.YNL267W

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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