pcm-1 Antibody

Code CSB-PA631678XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans pcm-1 Polyclonal antibody
Uniprot No.
Target Names
pcm-1
Alternative Names
pcm-1 antibody; C10F3.5 antibody; Protein-L-isoaspartate O-methyltransferase antibody; PIMT antibody; EC 2.1.1.77 antibody; L-isoaspartyl protein carboxyl methyltransferase antibody; Protein L-isoaspartyl methyltransferase antibody; Protein-beta-aspartate methyltransferase antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans pcm-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins.
Gene References into Functions
  1. the involvement of the insulin/insulin-like growth factor-1 signaling (IIS) pathway in PCM-1-dependent lifespan extension in C. elegans PMID: 21695191
  2. The longevity defects of pcm-1 mutants previously seen in dauer larvae and here in L1 larvae suggest a defect in the ability of pcm-1 mutants to recycle and reuse old cellular components in pathways such as autophagy. PMID: 17187774
  3. Autophagy and insulin/TOR signaling were studied in Caenorhabditis elegans pcm-1 protein repair mutants. PMID: 17404495
  4. pcm-1 deficient dauer larvae had reduced fat stores, suggesting that PCM-1 may have a role in the initiation of the correct metabolic responses to stress starvation. PMID: 18977240
  5. Report defective responses to oxidative stress in protein l-isoaspartyl repair-deficient Caenorhabditis elegans. PMID: 19682488

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Subcellular Location
Cytoplasm, cytosol.
Protein Families
Methyltransferase superfamily, L-isoaspartyl/D-aspartyl protein methyltransferase family
Database Links

STRING: 6239.C10F3.5a

UniGene: Cel.17987

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