PIN4 Antibody

Datasheet

Code	CSB-PA844580XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PIN4 Polyclonal antibody

Uniprot No.

Q8RWZ6

Target Names

PIN4

Alternative Names

PIN4 antibody; At2g01420 antibody; F2I9.4 antibody; Auxin efflux carrier component 4 antibody; AtPIN4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PIN4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Acts as a component of the auxin efflux carrier. Plays a role in generating a sink for auxin into columella cells. Maintains the endogenous auxin gradient, which is essential for correct root patterning. Involved in EXO70A3-regulated gravitropic responses in columella cells and in root system architecture (RSA).

Gene References into Functions

ABA treatment reduced the expression levels of the PIN-FORMEDs (PIN) auxin efflux carriers, PIN1, PIN3, PIN4, and PIN7, to a greater extent in the root meristems of arf2-101 mutant than in the wild type. ARF2 positively mediates the transcripts of transcription factor PLETHORA 1 (PLT1) gene but negatively mediates PLT2 at protein level in root meristems. PMID: 28074634
Functional interplay between protein kinase CK2 and salicylic acid sustains PIN4 transcriptional expression. PMID: 24547808
Data show that phototropic hypocotyl bending is strongly dependent on the activity of D6PKs and the PIN-FORMED (PIN) auxin efflux facilitators PIN3, PIN4, and PIN7. PMID: 23709629
NOV is required for cell type-specific expression of PIN4 in the root. PMID: 19880797
Data suggest that auxin transport activity, in particular that of the PIN-FORMED1 (PIN1) and PIN4 proteins, is a major factor in the maintenance of auxin gradients. PMID: 16055631
PP2A and PINOID both partially colocalize with PINs and act antagonistically on the phosphorylation state of their central hydrophilic loop, hence mediating PIN apical-basal polar targeting. PMID: 17889649

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Auxin efflux carrier (TC 2.A.69.1) family

Tissue Specificity

Expressed in the quiescent center precursors and surrounding cells. Present in columella cells of primary roots. Detected in pollen.

Database Links

KEGG: ath:AT2G01420

STRING: 3702.AT2G01420.2

UniGene: At.24881

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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PIN4 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background