PIN5 Antibody

Datasheet

Code	CSB-PA884219XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PIN5 Polyclonal antibody

Uniprot No.

Q9FFD0

Target Names

PIN5

Alternative Names

PIN5 antibody; AEH2 antibody; PIN8 antibody; At5g16530 antibody; MQK4.28 antibody; Auxin efflux carrier component 5 antibody; AtPIN5 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PIN5 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Auxin transporter regulating intracellular auxin homeostasis and metabolism. Mediates the auxin transport from the cytosol into the lumen of the endoplasmic reticulum. May also act as an auxin efflux carrier when located to the cell membrane. PIN5 and PIN8 may have an antagonistic/compensatory activity. Involved in unfolded protein response (UPR) activation. Involved in the control of vein patterning. Promotes vein formation. PIN5, PIN6, and PIN8 control vein network geometry, but they are expressed in mutually exclusive domains of leaf vascular cells.

Gene References into Functions

The functional relationship of IRE1 and PIN5 is demonstrated by that an ire1 pin5 triple mutant enhances defects of unfolded protein response (UPR) activation and auxin homeostasis in ire1 or pin5. PMID: 24180465
Arabidopsis thaliana PIN5, an atypical member of the PIN gene family, encodes a functional auxin transporter that is required for auxin-mediated development PMID: 19506555

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Protein Families

Auxin efflux carrier (TC 2.A.69.1) family

Tissue Specificity

Expressed in elongating parts of hypocotyl, cotyledon vasculature and guard cells. Detected in root pericycle and root tip and at later developmental stages in leaves, stems and flowers. Expressed in veins of mature leaves.

Database Links

KEGG: ath:AT5G16530

STRING: 3702.AT5G16530.1

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Phage Display Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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PIN5 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background