PIN8 Antibody

Datasheet

Code	CSB-PA873261XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PIN8 Polyclonal antibody

Uniprot No.

Q9LFP6

Target Names

PIN8

Alternative Names

PIN8 antibody; PIN5 antibody; At5g15100 antibody; F2G14_220 antibody; Auxin efflux carrier component 8 antibody; AtPIN8 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PIN8 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Component of the intracellular auxin-transport pathway in the male gametophyte. Involved in the regulation of auxin homeostasis in pollen. Involved in the efflux of auxin from the endoplasmic reticulum into the cytoplasm. PIN5 and PIN8 may have an antagonistic/compensatory activity. Involved in the control of vein patterning. Redundantly with PIN6, inhibits the vein-formation-promoting functions of PIN5. PIN5, PIN6, and PIN8 control vein network geometry, but they are expressed in mutually exclusive domains of leaf vascular cells.

Gene References into Functions

Data demonstrated a role for PIN8 in intracellular auxin homeostasis. PMID: 22990451
endoplasmic reticulum-localized PIN8 controls auxin thresholds and access of auxin to the nucleus, thereby regulating auxin-dependent transcriptional activity PMID: 22540348
ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis. PMID: 22760640

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Protein Families

Auxin efflux carrier (TC 2.A.69.1) family

Tissue Specificity

Expressed in veins of mature leaves. Strongly expressed in pollen.

Database Links

KEGG: ath:AT5G15100

STRING: 3702.AT5G15100.1

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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PIN8 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background