PIP2-1 Antibody

Code CSB-PA337370XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PIP2-1 Polyclonal antibody
Uniprot No.
Target Names
PIP2-1
Alternative Names
PIP2-1; PIP2A; At3g53420; F4P12.120; Aquaporin PIP2-1; Plasma membrane intrinsic protein 2-1; AtPIP2;1; Plasma membrane intrinsic protein 2a; PIP2a
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PIP2-1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Water channel required to facilitate the transport of water across cell membrane. Probably involved in root water uptake. Its function is impaired by Hg(2+).
Gene References into Functions
  1. Phosphorylation of AtPIP2;1 Ser121 by BAK1 and/or OST1 is triggered in response to flg22 to activate its water and H2O2 transport activities. PMID: 28784763
  2. PIP2;1 activity is regulated by by Ca(2+) and pH. PMID: 27620834
  3. study reveals that C-terminal phosphorylation of a water channel subunit, PIP2;1, is upregulated by an EIN3EIL1-independent ethylene signaling pathway and its water transport activity is enhanced by phosphorylation at the C-terminal S280 and S283 sites PMID: 26476206
  4. This work supports a model whereby abscissic acid-triggered stomatal closure requires an increase in guard cell permeability to water and possibly hydrogen peroxide, through OST1-dependent phosphorylation of PIP2;1 at Ser-121. PMID: 26163575
  5. PIP2;1 molecules are heterogeneously distributed on the plasma membrane and that clathrin and membrane raft pathways cooperate to mediate the subcellular trafficking of PIP2;1. PMID: 22010034
  6. Co-expression of AtPIP2;1-GFP and AtPIP1;4-mCherry constructs suggested that ER-retained AtPIP2;1-GFP may interact with other PIPs to hamper their trafficking to the PM, thereby contributing to inhibition of root cell hydraulic conductivity PMID: 21182578
  7. analysis of the subcellular trafficking of AtPIP2 PMID: 18234664
  8. Structure-function analysis of plant aquaporin AtPIP2. PMID: 18637793

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Note=A fuzzy intracellular localization is induced by salt (NaCl) treatment.
Protein Families
MIP/aquaporin (TC 1.A.8) family, PIP (TC 1.A.8.11) subfamily
Tissue Specificity
Predominantly expressed in roots and green siliques. Also expressed at lower level above ground and in flower buds.
Database Links

KEGG: ath:AT3G53420

STRING: 3702.AT3G53420.1

UniGene: At.47609

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