Product Details

Full Product Name

Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) piwil1 Polyclonal antibody

Uniprot No.

Q8UVX0

Target Names

piwil1

Alternative Names

piwil1 antibody; ziwi antibody; Piwi-like protein 1 antibody; EC 3.1.26.- antibody

Raised in

Rabbit

Species Reactivity

Danio rerio (Zebrafish) (Brachydanio rerio)

Immunogen

Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) piwil1 protein

Immunogen Species

Danio rerio (Zebrafish) (Brachydanio rerio)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Plays a central role during gametogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Has a strong preference for piRNAs with a uridine nucleotide at their 5'-end (g1U preference, also named 1U-bias) and binds piRNAs in an opposite direction compared to piwil2/zili. Participates in a piRNA amplification loop with piwil2/zili. Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs.

Gene References into Functions

the zebrafish homologue of the Drosophila piwi: co-localization with vasa at the embryonic genital ridge and gonad-specific expression in the adults (ziwi protein). PMID: 12617811
Tdrd1 acts as a molecular scaffold for Ziwi. PMID: 21743441
Promoter activity of a 4.8-kb sequence isolated from the 5' end of the zebrafish germ cell-specific ziwi gene is sufficient to drive gene expression in all germ cells with stage-specific differences in expression levels. PMID: 20737503
study demonstrated that zebrafish Piwi, Ziwi is expressed in gonad of both sexes and is a component of a structure called nuage; loss of Ziwi function results in a progressive loss of germ cells due to apoptosis during larval development PMID: 17418787
Ziwi and Zili bind piRNAs of opposite polarity PMID: 18833190

Hide All

Subcellular Location

Cytoplasm.

Protein Families

Argonaute family, Piwi subfamily

Tissue Specificity

Expressed exclusively in the adult gonads; expression in the ovary weaker than in the testis (at protein level). During neurogenesis and organogenesis, expression is detected in CNS (midbrain and eye) and fin buds. Starting from 24 hours post-fertilizatio

Database Links

KEGG: dre:368200

STRING: 7955.ENSDARP00000121147

UniGene: Dr.83448

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piwil1 Antibody

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