Product Details

Full Product Name

Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) piwil2 Polyclonal antibody

Uniprot No.

A2CEI6

Target Names

piwil2

Alternative Names

piwil2 antibody; zili antibody; si:dkey-88f5.1 antibody; Piwi-like protein 2 antibody; EC 3.1.26.- antibody

Raised in

Rabbit

Species Reactivity

Danio rerio (Zebrafish) (Brachydanio rerio)

Immunogen

Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) piwil2 protein

Immunogen Species

Danio rerio (Zebrafish) (Brachydanio rerio)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Plays an essential role in germ cell differentiation and meiosis, independently of the function in transposable elements repression. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' piwil4. Piwil2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by-product that is degraded. Required for piwil4/miwi2 nuclear localization and association with secondary piRNAs antisense. Represses circadian rhythms by promoting the stability and activity of core clock components ARNTL/BMAL1 and CLOCK.

Gene References into Functions

zili might have some effect on the formation of the ectoderm, mesoderm and endoderm during early embryogenesis PMID: 21265086
Tdrd1 binds both zebrafish Piwi proteins, Ziwi and Zili, and reveals sequence specificity in the interaction between Tdrd1 tudor domains and symmetrically dimethylated arginines (sDMAs) in Zili. PMID: 21743441
These results indicate that zili regulates dorsal-ventral patterning by antagonizing Bmp signaling during early embryogenesis in zebrafish. PMID: 21627449

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Argonaute family, Piwi subfamily

Tissue Specificity

Detected in primordial germ cells (PGCs) from 3 dpf. In adult, it is found in both the female and male gonad. In the ovary, it is present in all stages of germ cell differentiation. In testis, it is present in mitotic and meiotic germ cells. No protein ha

Database Links

KEGG: dre:572134

STRING: 7955.ENSDARP00000114406

UniGene: Dr.40934

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piwil2 Antibody

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