Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans vps-34 Polyclonal antibody

Uniprot No.

Q9TXI7

Target Names

vps-34

Alternative Names

vps-34 antibody; let-512 antibody; B0025.1 antibody; Phosphatidylinositol 3-kinase catalytic subunit type 3 antibody; PI3-kinase type 3 antibody; PI3K type 3 antibody; EC 2.7.1.137 antibody; Phosphoinositide-3-kinase class 3 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans vps-34 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Together with bec-1, mediates the production of phosphatidylinositol 3-phosphate on intracellular vesicles and thereby regulates membrane trafficking. Plays a role in endosome-to-Golgi retrograde transport of mig-14. Involved in clearance of apoptotic cell corpses by phagosomes. Phagosome maturation requires two sequential and non-overlapping pulses of phosphatidylinositol-3-phosphate (PI3P) on the vesicle surface which mediates recruitment of sortins snx-1 and lst-4 and small GTPases rab-5, rab-2 and rab-7, downstream of dynamin dyn-1. The first pulse is initiated by piki-1, then maintained by vps-34 which also produces the second pulse. Required for embryonic development. Together with bec-1, involved in L3/L4 larval molting stage probably by regulating cuticle shedding. Regulates the expansion of the nucleus outer membrane. Involved in the secretion and localization of lrp-1 at the apical surface of hyp7 syncytium. May regulate endocytosis in hypodermal cells. May play a role in the formation of gut granules (a lysosome-related organelle). Plays a role in germ stem cell proliferation during larval development.

Gene References into Functions

The data suggest that K63-linked ubiquitin modification serves as a general mechanism to modulate VPS-34 stability in multiple processes. PMID: 29092895
VPS-34 recruits TBC-2 to endosomes to inactivate RAB-5 to ensure the directionality of endosome maturation PMID: 28455411

Subcellular Location

Nucleus outer membrane; Peripheral membrane protein. Cytoplasm. Cytoplasmic granule. Cell projection, phagocytic cup.

Protein Families

PI3/PI4-kinase family

Tissue Specificity

Ubiquitous.

Database Links

KEGG: cel:CELE_B0025.1

STRING: 6239.B0025.1a

UniGene: Cel.6170

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vps-34 Antibody

Product Details

Customer Reviews and Q&A

Target Background