Product Details

Full Product Name

Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) pkd2 Polyclonal antibody

Uniprot No.

Q6IVV8

Target Names

pkd2

Alternative Names

pkd2 antibody; Polycystin-2 antibody; Curly up antibody; Cup antibody; Polycystic kidney disease 2 protein homolog antibody; Transient receptor potential cation channel subfamily P member 2 antibody

Raised in

Rabbit

Species Reactivity

Danio rerio (Zebrafish) (Brachydanio rerio)

Immunogen

Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) pkd2 protein

Immunogen Species

Danio rerio (Zebrafish) (Brachydanio rerio)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of a heteromeric calcium-permeable ion channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B. Can also form a functional, homotetrameric ion channel. Functions as non-selective, voltage-gated cation channel. Required for normal oscillation of Ca(2+) levels within cilia; these oscillations of the intraciliary Ca(2+) levels can trigger cytoplasmic Ca(2+) signaling cascades. May contribute to the release of Ca(2+) stores from the endoplasmic reticulum. Required for normal temporal variation of the intracellular Ca(2+) levels in the heart. Plays a role in fluid-flow mechanosensation. Required for normal specification of the body left-right axis during embryogenesis, most likely via its role in ciliary Ca(2+) oscillations in Kupffer's vesicle.

Gene References into Functions

Results show that Far Upstream Element-Binding Protein 1 Binds the 3' Untranslated Region of PKD2 to inhibit PKD2 translation, regulating zebrafish disease phenotypes associated with PKD2 knockdown PMID: 26839368
TRPP2 and TRPV4 are mechanosensitive channels in the endocardium.Oscillatory flow modulates mechanosensitive klf2a expression through trpv4 and trpp2 during heart valve development. PMID: 25959969
Intraciliary calcium oscillations depend on Pkd2 and are left-biased at the left-right organizer in response to ciliary motility. PMID: 25660539
TRPP2 utilizes TRPV4 to form a mechano- and thermosensitive molecular sensor in the cilium.[TRPP2] PMID: 18695040
Isolated pkd2 mutant hearts displayed impaired intracellular calcium cycling and calcium alternans. These results indicate heart failure in the pkd2 mutants. PMID: 23376035
PKD2 suppression inactivates CaMK-II in pronephric cells and cilia, whereas constitutively active CaMK-II restores pronephric duct formation in pkd2 morphants PMID: 21752935
Pkd1a/b and pkd2 interact to regulate extracellular matrix secretion or assembly, and that altered matrix integrity may be a primary defect underlying autosomal dominant polycystic kidney disease tissue pathologies. PMID: 20335443
atp-2, lov-1, and pkd2 act in the same molecular pathway. PMID: 15563610
The functions of polaris and pkd2 in LR patterning are conserved between zebrafish and mice and that Kupffer's vesicle PMID: 16216239
In zebrafish pkd2 is not responsible for the activation of spaw transcription, but is required for a mechanism to restrict spaw expression to the left half of the embryo. pkd2 also appears to play a role in the propagation of Nodal signals in the LPM. PMID: 17360770
role in the development of normal left-to-right asymmetry depends on endoplasmic reticulum localization PMID: 18417723

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Subcellular Location

Basolateral cell membrane; Multi-pass membrane protein. Cell membrane, sarcolemma. Cytoplasm, myofibril, sarcomere. Sarcoplasmic reticulum membrane. Apical cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell projection, cilium. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasmic vesicle membrane.

Protein Families

Polycystin family

Tissue Specificity

Detected along cilia and at the cilium basal body in Kupffer's vesicle at the 10 somite stage. Detected in heart at 48hpf. Detected in muscle and pronephric kidney at 48 hpf. Detected on trunk muscle sarcolemma and sarcomere, on ependymal cell cilia in br

Database Links

KEGG: dre:432387

STRING: 7955.ENSDARP00000027024

UniGene: Dr.92211

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pkd2 Antibody

Product Details

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Target Background