PKSA Antibody

Code CSB-PA589462XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PKSA Polyclonal antibody
Uniprot No.
Target Names
PKSA
Alternative Names
PKSA antibody; LAP6 antibody; At1g02050 antibody; T7I23.4 antibody; Type III polyketide synthase A antibody; PKS-A antibody; EC 2.3.1.- antibody; Hydroxyalkyl alpha-pyrone synthase PKS-A antibody; Protein LESS ADHESIVE POLLEN 6 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PKSA protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plant type III polyketide synthases (PKSs) that catalyzes the condensation of malonyl-CoA units with various CoA ester starter molecules to generate a diverse array of natural products including long-chain alkyl alpha-pyrones. Accepts up to C(20) chain-length fatty acyl CoAs as starter substrates, and carries out sequential condensations with malonyl-CoA to produce triketide and tetraketide alpha-pyrones, potential sporopollenin precursors. Favorite substrates for are midchain- and v-hydroxylated fatty acyl-CoAs (e.g. 12-hydroxyoctadecanoyl-CoA and 16-hydroxyhexadecanoyl-CoA). Required for pollen development and sporopollenin biosynthesis, the major constituent of exine in the outer pollen wall. In vitro, can use 4-coumaroyl-coenzyme A as substrate to produce bis-noryangonin and fatty acyl-coenzyme A as substrate to produce medium-chain alkyl pyrones. May play a role in both the synthesis of pollen fatty acids and phenolics found in exine.
Gene References into Functions
  1. At1g02050 interacts and constitutes a part of metabolon localized to the endoplasmic reticulum of tapetum cells.At1g02050 is sporopollenin biosynthetic enzyme. PMID: 23632852
  2. LAP5 and LAP6 are multifunctional enzymes and may play a role in both the synthesis of pollen fatty acids and phenolics found in exine.[LAP6] PMID: 20442277
Subcellular Location
Endoplasmic reticulum.
Protein Families
Chalcone/stilbene synthases family
Tissue Specificity
Expressed in flowers and flower buds (at protein level), and, at very low levels, in roots, seedlings, leaves and stems. Mostly confined to anther tapetal cells.
Database Links

KEGG: ath:AT1G02050

STRING: 3702.AT1G02050.1

UniGene: At.43436

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