Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PKSB Polyclonal antibody

Uniprot No.

Q8LDM2

Target Names

PKSB

Alternative Names

PKSB antibody; LAP5 antibody; At4g34850 antibody; T11I11.90 antibody; Type III polyketide synthase B antibody; PKS-B antibody; EC 2.3.1.- antibody; Hydroxyalkyl alpha-pyrone synthase PKS-B antibody; Protein LESS ADHESIVE POLLEN 5 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PKSB protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Plant type III polyketide synthases (PKSs) that catalyzes the condensation of malonyl-CoA units with various CoA ester starter molecules to generate a diverse array of natural products including long-chain alkyl alpha-pyrones. Accepts up to C(20) chain-length fatty acyl CoAs as starter substrates, and carries out sequential condensations with malonyl-CoA to produce triketide and tetraketide alpha-pyrones, potential sporopollenin precursors. Favorite substrates for are midchain- and v-hydroxylated fatty acyl-CoAs (e.g. 12-hydroxyoctadecanoyl-CoA and 16-hydroxyhexadecanoyl-CoA). Required for pollen development and sporopollenin biosynthesis, the major constituent of exine in the outer pollen wall. In vitro, can use 4-coumaroyl-coenzyme A as substrate to produce bis-noryangonin and fatty acyl-coenzyme A as substrate to produce medium-chain alkyl pyrones. May play a role in both the synthesis of pollen fatty acids and phenolics found in exine.

Gene References into Functions

AT4G34850 interacts and constitutes a part of metabolon localized to the endoplasmic reticulum of tapetum cells.AT4G34850 is sporopollenin biosynthetic enzyme. PMID: 23632852
results show hydroxylated alpha-pyrone polyketide compounds generated by the sequential action of ACOS5 and LAP6/LAP5 are potential and previously unknown sporopollenin precursors PMID: 21193570
LAP5 and LAP6 are multifunctional enzymes and may play a role in both the synthesis of pollen fatty acids and phenolics found in exine.[LAP5] PMID: 20442277

Subcellular Location

Endoplasmic reticulum.

Protein Families

Chalcone/stilbene synthases family

Tissue Specificity

Expressed in flowers and flower buds (at protein level). Mostly confined to anther tapetal cells.

Database Links

KEGG: ath:AT4G34850

STRING: 3702.AT4G34850.1

UniGene: At.3503

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PKSB Antibody

Product Details

Customer Reviews and Q&A

Target Background