PLA2-ALPHA Antibody

Datasheet

Code	CSB-PA200636XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PLA2-ALPHA Polyclonal antibody

Uniprot No.

Q8S8N6

Target Names

PLA2-ALPHA

Alternative Names

PLA2-ALPHA antibody; At2g06925 antibody; T4E14.19 antibody; Phospholipase A2-alpha antibody; EC 3.1.1.4 antibody; Secretory phospholipase A2-alpha antibody; AtsPLA2-alpha antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PLA2-ALPHA protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Releases lysophospholipids (LPLs) and free fatty acids (FFAs) from membrane phospholipids in response to hormones and other external stimuli. Modulates the trafficking of PIN proteins to the plasma membrane. Negatively regulates MYB30 transcriptional activity and hypersensitive response control.

Gene References into Functions

Extended loop structures at the N-terminus in AtPLA2alpha are suggested to be the main reasons for the much lower thermodynamic stability compared with its homologues. PMID: 24389456
These results indicate that pPLAIIalpha negatively regulates oxylipin production and suggest a role in the removal of oxidatively modified fatty acids from membranes. PMID: 22259021
AtMYB30 and the secreted phospholipase AtsPLA(2)-alpha physically interact in vivo, following the AtMYB30-mediated specific relocalization of AtsPLA(2)-alpha from cytoplasmic vesicles to the plant cell nucleus. PMID: 20696912
analysis of secretory phospholipase A2-alpha from Arabidopsis thaliana PMID: 17692835

Subcellular Location

Secreted. Golgi apparatus. Cytoplasmic vesicle. Nucleus.

Protein Families

Phospholipase A2 family

Tissue Specificity

Ubiquitous but expressed at a low level.

Database Links

KEGG: ath:AT2G06925

STRING: 3702.AT2G06925.1

UniGene: At.23179

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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