PLA2-DELTA Antibody

Datasheet

Code	CSB-PA121257XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PLA2-DELTA Polyclonal antibody

Uniprot No.

Q8GV50

Target Names

PLA2-DELTA

Alternative Names

PLA2-DELTA antibody; At4g29470 antibody; F17A13.290 antibody; Phospholipase A2-delta antibody; EC 3.1.1.4 antibody; Secretory phospholipase A2-delta antibody; AtsPLA2-delta antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PLA2-DELTA protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Releases lysophospholipids (LPLs) and free fatty acids (FFAs) from membrane phospholipids in response to hormones and other external stimuli. Plays a role in pollen development and germination and tube growth.

Gene References into Functions

Data describe the cloning and functional characterization of a phospholipase (PL)A2 cDNA from Arabidopsis thaliana, AtsPLA2-alpha, which represents one of four secretory PLA2 genes in Arabidopsis. PMID: 16140037
Mutagenesis of amino acids supposed to be involved in catalysis has shown that His62, the calcium-coordinating Asp63, and the above-mentioned Ser79 residue are essential for activity PMID: 16669612
Rapid auxin-regulated gene expression was tested for sensitivity to PLA(2) inhibitors using seedlings expressing beta-glucuronidase (GUS) under the control of the synthetic auxin-responsive promoter DR5. PMID: 17692850

Subcellular Location

Secreted. Endoplasmic reticulum.

Protein Families

Phospholipase A2 family

Tissue Specificity

Specifically expressed in flowers but at a low level. Detected specifically in the pollen.

Database Links

KEGG: ath:AT4G29470

STRING: 3702.AT4G29470.1

UniGene: At.31918

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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