PLC2 Antibody

Code CSB-PA657184XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PLC2 Polyclonal antibody
Uniprot No.
Target Names
PLC2
Alternative Names
PLC2 antibody; At3g08510 antibody; T8G24.4 antibody; Phosphoinositide phospholipase C 2 antibody; EC 3.1.4.11 antibody; Phosphoinositide phospholipase PLC2 antibody; AtPLC2 antibody; PI-PLC2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PLC2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. At physiological calcium concentration, the preferred substrate is phosphatidylinositol 4,5-bisphosphate versus phosphatidylinositol.
Gene References into Functions
  1. The generation of reactive oxygen species (ROS) upon flg22 treatment is compromised in the PLC2-defficient plants, suggesting an effect of PLC2 in a branch of MAMP-triggered immunity and nonhost resistance that involves early ROS-regulated processes. Consistently, PLC2 associates with the NADPH oxidase RBOHD, suggesting its potential regulation by PLC2. PMID: 28827453
  2. essential for female gametogenesis and embryo development PMID: 27999988
  3. PLC2 is involved in auxin biosynthesis and signaling, thus modulating development of both male and female gametophytes in Arabidopsis. PMID: 26340337
  4. seedlings of plc2-1 mutant showed growth defect that was complemented by heterologous expression of AtPLC2, suggesting that phosphoinositide-specific phospholipase C activity borne by AtPLC2 is required for seedling growth PMID: 26401841
  5. AtPI4KIIIbeta1 and AtPI4KIIIbeta2 can both act upstream of the PI-PLC. There is evidence for the occurrence of a de novo synthesis of PtdIns4P by PI4K that coincides with PI-PLC activation. [PI-PLC] PMID: 22318862

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Subcellular Location
Cell membrane; Peripheral membrane protein.
Tissue Specificity
Expressed in roots, shoots, leaves and flowers.
Database Links

KEGG: ath:AT3G08510

STRING: 3702.AT3G08510.1

UniGene: At.20554

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