Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans plc-1 Polyclonal antibody

Uniprot No.

G5EFI8

Target Names

plc-1

Alternative Names

plc-1 antibody; F31B12.1 antibody; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 antibody; EC 3.1.4.11 antibody; Phosphoinositide phospholipase C-epsilon plc-1 antibody; Phosphoinositide-specific phospholipase PLC210 antibody; Phospholipase C-epsilon plc-1 antibody; PLC-epsilon plc-1 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans plc-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. plc-1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. By activating IP3 receptor itr-1-mediated intracellular Ca(2+) release via the production of IP3, regulates ovulation by controlling contraction and/or dilation of the distal spermatheca valve during oocyte entry and the timing of the dilation of the spermatheca-uterine valve during oocyte exit. In a similar manner, plays an essential role in epidermal morphogenesis by regulating migration of epidermal cells during ventral closure and to a lesser extent by regulating epidermal cell dorsal intercalation. Involved in the immune response to S.aureus bacterium by activating kinase dkf-1 via the production of DAG which in turn activates transcription factor hlh-30. In ASER neurons, required for adjusting the orientation behavior in salt gradients based on the memory of previous salt concentration encountered.

Gene References into Functions

Using genetic analysis and a calcium-sensitive fluorescent protein, we show that FLN-1 functions with PLC-1 to regulate IP3 production, calcium release, and contraction of the spermatheca PMID: 23671426
Phospholipase Cepsilon (plc-1)regulates ovulation in Caenorhabditis elegans. PMID: 15355798

Tissue Specificity

Expressed in the spermatheca, vulva, intestine and excretory cells. Expressed in sensory neurons AWC, AFD, ASE, ASG and BAG, interneurons, ventral nerve cord neurons and tail neurons. Expressed in body muscles.

Database Links

KEGG: cel:CELE_F31B12.1

STRING: 6239.F31B12.1a

UniGene: Cel.22953

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plc-1 Antibody

Product Details

Customer Reviews and Q&A

Target Background