PLDALPHA1 Antibody

Code CSB-PA656435XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PLDALPHA1 Polyclonal antibody
Uniprot No.
Target Names
PLDALPHA1
Alternative Names
PLDALPHA1 antibody; PLD1 antibody; At3g15730 antibody; MSJ11.13 antibody; Phospholipase D alpha 1 antibody; AtPLDalpha1 antibody; PLD alpha 1 antibody; EC 3.1.4.4 antibody; Choline phosphatase 1 antibody; PLDalpha antibody; Phosphatidylcholine-hydrolyzing phospholipase D 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PLDALPHA1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond to generate phosphatidic acids (PA). Plays an important role in various cellular processes, including phytohormone action and response to stress, characterized by acidification of the cell. Involved in wound induction of jasmonic acid. May be involved in membrane lipid remodeling. Probably involved in freezing tolerance by modulating the cold-responsive genes and accumulation of osmolytes. Can use phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) as substrates, both in presence or in absence of PIP2. Its main substrate is phosphatidylcholine. Stimulates the intrinsic GTPase activity of GPA1 upon binding. Mediates the abscisic acid effects on stomata through interaction with GPA1 and the production of phosphatidic acid that bind to ABI1. Involved in seed aging and deterioration. Involved in microtubule stabilization and salt tolerance. Involved in abscisic acid-induced stomatal closure.
Gene References into Functions
  1. PLDalpha1-mediated phosphatidic acid formation modulates desiccation sensitivity as applying its inhibitor improved seed desiccation tolerance and its suppression in protoplasts enhanced survival under dehydration. PMID: 28152567
  2. In Arabidopsis thaliana, PLDalpha1/PA might regulate cell development by modulating microtubule organization in an activity-dependent manner. PMID: 27864640
  3. The phospholipase Dalpha1 protein is a key component and modulator of the G-protein complex in affecting a subset of signaling pathways. PMID: 26935351
  4. PLDalpha1 and phosphatidic acid regulate microtubular organization and Ca(2+) increases during abscisic acid (ABA)-induced stomatal closing and that crosstalk among signaling lipid, Ca(2+), and microtubules is essential for ABA signaling. PMID: 24271006
  5. Ablation of guard cell-expressed copper amine oxidase gamma or phospholipase Dalpha1 gene retarded abscisic acid-induced H2O2 generation and stomatal closure. PMID: 24817219
  6. ACBP1 interacts with PLDalpha1 at the plasma membrane. Their interaction was further confirmed by yeast two-hybrid analysis. As recombinant ACBP1 binds phosphatidic acid and phosphatidylcholine, ACBP1 probably promotes PLDalpha1 action. PMID: 23448237
  7. PLDalpha1-derived phosphatidic acid (PA) binds to MAP65-1, thus mediating microtubule stabilization and salt tolerance. It reveals a functional connection between membrane lipids and the cytoskeleton in environmental stress signaling. PMID: 23150630
  8. PLDalpha1 and PLDdelta are involved in reactive oxygen species and nitric oxide production and cytosolic alkalization. PLDalpha1 and PLDdelta cooperate in ABA signaling in guard cells. PMID: 22392280
  9. SPHK/phyto-S1P and PLDalpha1A are co-dependent in amplification of response to ABA, mediating stomatal closure in Arabidopsis. PMID: 22275366
  10. Piriformospora indica-stimulated growth response is mediated by a pathway consisting of the PLD-PDK1-OXI1 cascade. PMID: 21625539
  11. PLDalpha1 mediates the abscisic acid effects on stomata through interaction with a protein phosphatase 2C & a heterotrimeric GTP-binding protein PMID: 16614222
  12. PLDalpha1 plays a key role in freezing tolerance of Arabidopsis by modulating the cold-responsive genes and accumulation of osmolytes. PMID: 16949955
  13. Identification of PLDalpha-mediated events in response to progressive drought stress in Arabidopsis is reported. PMID: 17261695
  14. A high level of PLDalpha1 is detrimental to seed quality, and attenuation of PLDalpha1 expression has the potential to improve oil stability, seed quality and seed longevity. PMID: 17565616
  15. Report show that the presence of PLDalpha1 correlates with phospholipid hydrolysis in both freezing and post-freezing phases, suggesting a negative role for PLDalpha1 in freezing tolerance.. PMID: 17962199
  16. AtPLDalpha1 is activated after wounding but is restricted to ruptured cells. PMID: 19220780
  17. The pldalpha1 mutant failed to produce ROS in guard cells in response to ABA. PMID: 19690149

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Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein. Mitochondrion membrane. Microsome membrane. Vacuole. Cytoplasmic vesicle, clathrin-coated vesicle.
Protein Families
Phospholipase D family, C2-PLD subfamily
Tissue Specificity
Highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in seeds.
Database Links

KEGG: ath:AT3G15730

STRING: 3702.AT3G15730.1

UniGene: At.23882

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