Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PLDDELTA Polyclonal antibody

Uniprot No.

Q9C5Y0

Target Names

PLDDELTA

Alternative Names

PLDDELTA antibody; At4g35790 antibody; F4B14.60 antibody; Phospholipase D delta antibody; AtPLDdelta antibody; PLD delta antibody; EC 3.1.4.4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PLDDELTA protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond to generate phosphatidic acids (PA). May be involved in PA accumulation in the dehydration stress response and in the transduction of hormonal and environmental signals to the microtubules cytoskeleton. Prefers phosphatidylethanolamine to phosphatidylcholine as substrate. Involved in H(2)O(2) and abscisic acid (ABA)-induced stomatal closure. Involved in nitric oxide (NO) signaling during stomatal closure. Plays a positive role in ABA-promoted senescence. Involved in basal defense and nonhost resistance.

Gene References into Functions

PLDdelta negatively regulates plant thermotolerance via destabilizing cortical microtubules. PMID: 28710795
in wt plants PLDdelta disrupt membranes in severe drought stress and, in the absence of the protein (PLDdelta knock-out) might drought-prime the plants, making them more tolerant to severe drought stress. PMID: 26340512
PLD is involved in defense signaling in nonhost resistance against powdery mildew fungi and PLDdelta is the main isoform participating in this process. PMID: 23979971
PLDdelta-mediated hydrolysis of phosphatidylcholine to phosphatidic acid plays a positive role in abscisic acid-promoted senescence. PMID: 23762411
Phospholipase Ddelta is downstream of nitric oxide and hydrogen peroxide in abscisic acid (ABA)-induced stomatal closure. PMID: 22932846
Report show that the presence of PLDdelta correlates with reduced lipid hydrolysis during post-freezing recovery, suggesting a positive role for PLDdelta in freezing tolerance. PMID: 17962199

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Subcellular Location

Cell membrane; Peripheral membrane protein.

Protein Families

Phospholipase D family, C2-PLD subfamily

Tissue Specificity

Expressed in roots, leaves, stems, siliques and flowers. Strongly expressed in the vascular tissues of cotyledons and leaves under dehydration stress conditions. Expression is higher in old leaves than in young leaves. Expressed in leaves and guard cells.

Database Links

KEGG: ath:AT4G35790

STRING: 3702.AT4G35790.1

UniGene: At.22959

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PLDDELTA Antibody

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