Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PLPZETA2 Polyclonal antibody

Uniprot No.

Q9M9W8

Target Names

PLPZETA2

Alternative Names

PLPZETA2 antibody; PLDP2 antibody; At3g05630 antibody; F18C1.10 antibody; Phospholipase D zeta 2 antibody; PLDzeta2 antibody; EC 3.1.4.4 antibody; Phospholipase D p2 antibody; AtPLDp2 antibody; Phospholipase D2 PHOX and PX-containing domain protein antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PLPZETA2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond to generate phosphatidic acids (PA). Phosphatidylcholine-selective. Regulates vesicle trafficking and auxin responses. Required for the normal cycling of PIN-2 containing vesicles. Contributes to the supply of inorganic phosphorus for cell metabolism and diacylglycerol moieties for galactolipid synthesis in phosphorus-starved roots. Involved in root elongation during phosphate limitation.

Gene References into Functions

The efficiency of photosystem II, estimated by measuring the ratio of chlorophyll fluorescence (F v/ m ratio), was strongly decreased in pldzeta1 under salt stress. In conclusion, PLDzeta2 plays a key role in determining Arabidopsis sensitivity to salt stress allowing ion transport and antioxidant responses to be finely regulated. PMID: 28667438
AtPLDzeta2 is involved in phosphatidic acids production by a calcium signaling pathway, while AtPLDzeta1 is more important in reactive oxygen species signaling. PMID: 28834646
A transcriptional enhancer is present in the PLDZ2 promoter and able to confer Pi responsiveness to a minimal, inactive 35S promoter. This enhancer also shares the cytokinin and sucrose responsive properties observed for the intact PLDZ2 promoter. PMID: 22210906
A variant of the PHR1 binding site is highly enriched in the Arabidopsis phosphate-responsive phospholipase DZ2 coexpression network. PMID: 22836502
PLDzeta2 responds to drought through ABA signaling in the root cap and accelerates root hydrotropism through the suppression of root gravitropism. PMID: 19915862
PLDzeta2 plays a role in regulating root development in response to nutrient limitation. PMID: 16384909
pldz2 mutant is defective in the hydrolysis of phospholipids and has a reduced capacity to accumulate galactolipids under limiting Pi conditions PMID: 16617110
Hydrolysis of phosphatidylcholine by PLD zetas during phosphorus starvation contributes to the supply of inorganic phosphorus in A. thaliana. PMID: 16891548
PLDzeta2 and and its product, phosphatidic acid (PA) are required for the normal cycling of PIN2-containing vesicles as well as for function in auxin transport and distribution, and hence auxin responses. PMID: 17259265
AtPLDzeta2 localizes to the tonoplast and the Nter regulatory domain is sufficient for its sorting. Under phosphate deprivation, AtPLDzeta2 remains located in the tonoplast but its distribution is uneven. PMID: 18242181

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Protein Families

Phospholipase D family, PXPH-PLD subfamily

Tissue Specificity

Expressed in seedlings, roots, leaves, stems and flowers. Highest expression in roots. Detected only in the meristematic regions up to 4 days after germination and then at later stages in all tissues.

Database Links

KEGG: ath:AT3G05630

STRING: 3702.AT3G05630.1

UniGene: At.40640

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PLPZETA2 Antibody

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