plk-2 Antibody

Code CSB-PA863089XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans plk-2 Polyclonal antibody
Uniprot No.
Target Names
plk-2
Alternative Names
plk-2 antibody; Y71F9B.7 antibody; Serine/threonine-protein kinase plk-2 antibody; EC 2.7.11.21 antibody; Polo-like kinase 2 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans plk-2 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Serine/threonine-protein kinase which plays a role, during oogenesis, in chromosome pairing and synapsis, by facilitating the recruitment and attachment of meiotic chromosomes to the nuclear envelope during prophase. Promotes the localization of brc-1 to the short arm of homologous chromosomes during meiotic prophase I. Regulates the formation of sun-1 patches along the nuclear envelope. Promotes meiotic nuclei apoptosis in response to chromosomal asynapsis. Plays a redundant role with plk-1 in the establishment of cell polarity downstream of mex-5 and mex-6 during the first embryonic cell divisions. Plays a role in nicotinic acetylcholine receptor-mediated sensitivity to nicotine but not levamisole. Regulates motility.
Gene References into Functions
  1. The authors found that SYP-4 is phosphorylated dependent on Polo-like kinases PLK-1/2. They propose a model in which Polo-like kinases recognize crossover designation and phosphorylate SYP-4 thereby stabilizing the synaptonemal complex and making chromosomes less permissive for further double-strand break formation. PMID: 28346135
  2. Here we show that CHK-2 promotes pairing and synapsis by phosphorylating a family of zinc finger proteins that bind to specialized regions on each chromosome known as pairing centers, priming their recruitment of the Polo-like kinase PLK-2. PMID: 26506311
  3. Polo kinases, via their polo box domains, bind to and regulate the activity of two key polarity proteins, MEX-5 and MEX-6. PMID: 18199581
Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Chromosome.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, CDC5/Polo subfamily
Tissue Specificity
Expressed in oocytes.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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