SAK Antibody

Code CSB-PA018196XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) SAK Polyclonal antibody
Uniprot No.
Target Names
SAK
Alternative Names
SAK antibody; CG7186 antibody; Serine/threonine-protein kinase PLK4 antibody; EC 2.7.11.21 antibody; Polo-like kinase 4 antibody; PLK-4 antibody; Serine/threonine-protein kinase SAK antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) SAK protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the mother centriole cylinder, using mother centriole as a platform, leading to the recruitment of centriole biogenesis proteins such as Sas-6. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Centrosome amplification following overexpression can initiate tumorigenesis, highlighting the importance of centrosome regulation in cancers.
Gene References into Functions
  1. Plk4 phosphorylates Cep135 to properly position the essential centriole component Asterless. PMID: 27558293
  2. Plk4 phosphorylates Ana2 at S38 and STAN motif and promotes Ana2 recruitment to the centriole. PMID: 29263250
  3. When Asl reduction is attenuated by Asl overexpression, plk4 mutations, Plk4 RNAi, or Slimb overexpression, Asl levels are higher in spermatozoa, resulting in embryos with reduced viability. PMID: 26480844
  4. Drosophila Plk4 phosphorylates four conserved serines in the STAN motif of the core centriole protein Ana2 to enable it to bind and recruit its Sas6 partner. PMID: 25264260
  5. Plk4 directly generates its own phosphodegron.Phosphorylation of only S293 of the Slimb-recognition motif is required for Slimb binding to Plk4. PMID: 24184097
  6. Regulation of autophosphorylation controls PLK4 self-destruction and centriole number.PLK4 protein levels are controlled by Slimb. PMID: 24184099
  7. SAK/PLK4 is required for centriole duplication and flagella development. PMID: 16326102

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Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, CDC5/Polo subfamily
Tissue Specificity
Expressed in testis (at protein level).
Database Links

KEGG: dme:Dmel_CG7186

STRING: 7227.FBpp0078042

UniGene: Dm.3240

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