Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AHA2 Polyclonal antibody

Uniprot No.

P19456

Target Names

AHA2

Alternative Names

AHA2 antibody; At4g30190 antibody; F9N11.40 antibody; ATPase 2 antibody; plasma membrane-type antibody; EC 7.1.2.1 antibody; Proton pump 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AHA2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

The plasma membrane H(+) ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses. Involved in maintaining the membrane potential and delta-pH, together forming the plasma membrane protonmotive force (PMF) required for root and hypocotyl elongation and root tropism. Important for root growth and development during different nitrogen regimes. Forms a functional cation-translocating unit with CNGC17 that is activated by PSKR1/BAK1 and possibly other BAK1/RLK complexes.

Gene References into Functions

The accumulation of AHA2 in intracellular compartments is consistent with reduced H(+) secretion near the transition zone and the suppression of root growth. PMID: 29042459
the PM proton pump AHA2 (Arabidopsis plasma membrane H(+) -ATPase isoform 2) is important for root growth and development during different nitrogen regimes. PMID: 25382626
AHA2 is activated by lysophospholipids directly via a mechanism involving N-and C- terminal domains. PMID: 25971968
analysis of Arabidopsis thaliana plasma membrane H(+)-ATPase isoform 2 reconstituted into soluble nanoscale lipid bilayers, also termed nanodiscs PMID: 23836891
Asn-106 is important for closure of the proton entrance pathway prior to proton translocation across the membrane in AHA2 PMID: 23420846
ABA has no effect on blue light-induced phosphorylation of the H+-ATPase in ABA-insensitive mutants PMID: 21666226
AHA2 and Na+,K+-ATPase crystals were both highly fragile and sensitive to manipulation, resulting in only low-resolution heavy-atom derivative data sets being available for phasing (6-9 A degrees maximum resolution). PMID: 20179343
first structure of a P-type proton pump (AHA2) determined by X-ray crystallography PMID: 18075595
the rhizosphere acidification in response to Fe deficiency is chiefly mediated by AHA2... PMID: 19549134

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIIA subfamily

Tissue Specificity

Higher levels in roots than in shoots. Expressed in epidermal and root cortex cells, in phloem, xylem and root hairs. Detected in cotyledons, hypocotyls, roots and root hairs.

Database Links

KEGG: ath:AT4G30190

STRING: 3702.AT4G30190.2

UniGene: At.23014

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AHA2 Antibody

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