Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PME3 Polyclonal antibody

Uniprot No.

O49006

Target Names

PME3

Alternative Names

PME3 antibody; ARATH27 antibody; At3g14310 antibody; MLN21.10 antibody; Pectinesterase/pectinesterase inhibitor 3 [Includes: Pectinesterase inhibitor 3 antibody; Pectin methylesterase inhibitor 3); Pectinesterase 3 antibody; PE 3 antibody; EC 3.1.1.11 antibody; Pectin methylesterase 27 antibody; AtPME27 antibody; Pectin methylesterase 3)] antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PME3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Acts in the modification of cell walls via demethylesterification of cell wall pectin.

Gene References into Functions

alters the metabolism of cruciferin seed storage proteins during post-germinative growth of seedlings PMID: 28375469
Docking analysis indicated that the inhibition of AtPME3 could occur via the interaction of AtPMEI7 with a PME ligand-binding cleft structure. PMID: 26183897
Transgenic lines overexpressing the DUF642 proteins encoded by At4g32460 and At5g11420 showed increased pectin methyl esterase 3 activity during seed germination, and improved seed germination performance. PMID: 25442819
role of pectin methyl esterase and preference in wall-associated kinase-activated, EDS1/PAD4-dependent stress response in Arabidopsis PMID: 24855660
Ectopic expression of AtPME3 causes strong Zn(2+) hypersensitivity that is tightly correlated with transcript abundance. Together these observations suggest detrimental effects on Golgi-localized processes. PMID: 23826687
The biochemical characterization of the Arabidopsis thaliana pectin methylesterase 3 gene (AtPME3; At3g14310) and its role in plant development, is reported.[PME3] PMID: 21692803
Upon infection, Pectobacterium carotovorum and Botrytis cinerea induce in Arabidopsis a rapid expression of AtPME3 that acts as a susceptibility factor and is required for the initial colonization of the host tissue. PMID: 21171891
PME3 directly interacts with the nematode cellulose binding protein which aids cyst nematode parasitism. PMID: 19001564

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Subcellular Location

Secreted, extracellular space, apoplast. Secreted, cell wall.

Protein Families

PMEI family; Pectinesterase family

Tissue Specificity

Expressed in leaves, stems, flowers and siliques.

Database Links

KEGG: ath:AT3G14310

STRING: 3702.AT3G14310.1

UniGene: At.43283

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PME3 Antibody

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Target Background