POMGNT2 Antibody

Code CSB-PA847672XA01HU
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) POMGNT2 Polyclonal antibody
Uniprot No.
Target Names
POMGNT2
Alternative Names
POMGNT2 antibody; AGO61 antibody; C3orf39 antibody; EOGTL antibody; GTDC2 antibody; Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 antibody; POMGnT2 antibody; EC 2.4.1.312 antibody; Extracellular O-linked N-acetylglucosamine transferase-like antibody; Glycosyltransferase-like domain-containing protein 2 antibody
Raised in
Rabbit
Species Reactivity
Homo sapiens (Human)
Immunogen
Recombinant Homo sapiens (Human) POMGNT2 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
O-linked mannose beta-1,4-N-acetylglucosaminyltransferase that transfers UDP-N-acetyl-D-glucosamine to the 4-position of the mannose to generate N-acetyl-D-glucosamine-beta-1,4-O-D-mannosylprotein. Involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity.
Gene References into Functions
  1. POMGNT2 when two of the conserved amino acids are replaced. These findings begin to define the selectivity of POMGNT2 and suggest that this enzyme functions as a gatekeeper enzyme to prevent the vast majority of O-mannosylated sites on proteins from becoming modified with glycan structures functional for binding laminin globular domain-containing proteins PMID: 27932460
  2. GTDC2 generates CTD110.6 antibody-reactive N-acetylglucosamine epitopes on the O-mannosylated alpha-dystroglycan. PMID: 24041696
  3. Using WES in consanguineous WWS-affected families, we found multiple deleterious mutations in GTDC2 (also known as AGO61). . PMID: 22958903
Involvement in disease
Muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies A8 (MDDGA8)
Subcellular Location
Endoplasmic reticulum membrane; Single-pass type II membrane protein.
Protein Families
Glycosyltransferase 61 family
Tissue Specificity
Highly expressed in the brain, muscle, heart, and kidney in both fetus and adult. In the brain, highest expression in the cortex and cerebellum. Highly expressed in the pancreas.
Database Links

HGNC: 25902

OMIM: 614828

KEGG: hsa:84892

STRING: 9606.ENSP00000344125

UniGene: Hs.745002

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