PNSL5 Antibody

Code CSB-PA857684XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PNSL5 Polyclonal antibody
Uniprot No.
Target Names
PNSL5
Alternative Names
PNSL5 antibody; CYP20-2 antibody; At5g13120 antibody; T19L5_80 antibody; Photosynthetic NDH subunit of lumenal location 5 antibody; chloroplastic antibody; Cyclophilin of 20 kDa 2 antibody; Peptidyl-prolyl cis-trans isomerase CYP20-2 antibody; PPIase CYP20-2 antibody; EC 5.2.1.8 antibody; Rotamase CYP20-2 antibody; Thylakoid lumen PPIase of 20 kDa antibody; TLP20 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PNSL5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient (Probable). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Responsible, with FKBP13, for all PPIase activity observed in thylakoid lumen. Modulates the conformation of BZR1, which regulates flowering.
Gene References into Functions
  1. findings show that AtCYP20-2 is associated with a thylakoid protein complex, the NAD(P)H dehydrogenase (NDH) complex PMID: 19549520
  2. Double mutant plants disrupted AtCYP20-2 and AtFKBP13 did not show any phenotypic changes compared with wild-type plants, while peptidyl-prolyl isomerase activity activity was almost completely abolished from the mutant thylakoid lumen. PMID: 19717822
Subcellular Location
Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side.
Protein Families
Cyclophilin-type PPIase family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: ath:AT5G13120

STRING: 3702.AT5G13120.1

UniGene: At.10074

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