Product Details

Full Product Name

Rabbit anti-Rabbit hemorrhagic disease virus (strain Rabbit/Germany/FRG/1989) (Ra/LV/RHDV/GH/1989/GE) (RHDV-FRG) ORF1 Polyclonal antibody

Uniprot No.

P27410

Target Names

ORF1

Alternative Names

ORF1 antibody; Genome polyprotein antibody; p254) [Cleaved into: Protein p16; Protein p23; NTPase antibody; EC 3.6.1.15 antibody; 2C-like protein antibody; P2C antibody; p37); Precursor p41; Protein p29; Protein p23/2; Protein p18; Viral genome-linked protein antibody; VPg antibody; p13); 3C-like protease antibody; 3CLpro antibody; EC 3.4.22.66 antibody; Calicivirin antibody; Thiol protease P3C antibody; p15); RNA-directed RNA polymerase antibody; EC 2.7.7.48 antibody; 3Dpol antibody; p58); Capsid protein VP60] antibody

Raised in

Rabbit

Species Reactivity

Rabbit hemorrhagic disease virus (strain Rabbit/Germany/FRG/1989) (Ra/LV/RHDV/GH/1989/GE) (RHDV-FRG)

Immunogen

Recombinant Rabbit hemorrhagic disease virus (strain Rabbit/Germany/FRG/1989) (Ra/LV/RHDV/GH/1989/GE) (RHDV-FRG) ORF1 protein

Immunogen Species

Rabbit hemorrhagic disease virus (strain Rabbit/Germany/FRG/1989) (Ra/LV/RHDV/GH/1989/GE) (RHDV-FRG)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

NTPase presumably plays a role in replication.; Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation.; 3C-like protease processes the polyprotein: 3CLpro-RdRp (p72) is first released by autocleavage, then all other proteins are cleaved.; RNA-directed RNA polymerase replicates genomic and antigenomic RNA by recognizing replications specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs.; Capsid protein VP60 self assembles to form an icosahedral capsid with a T=3 symmetry, about 35 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate VP2 proteins and genomic or subgenomic RNA. Attaches virion to target cells by binding histo-blood group antigens, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm.

Gene References into Functions

study identified a putative functional motif that influences the subcellular localisation of the RHDV RdRp and its ability to interfere with the organisation of the Golgi apparatus PMID: 28763035
VPg serves as a novel cap substitute during the initiation of RHDV translation PMID: 26599265
VP1 with an epitope insertion at the N terminus assembled into mixtures of T=3 and larger T=4 capsids. PMID: 22491457
Three codons showed signs of positive selection in the major capsid protein VP60. PMID: 18761043
VP2 is not essential for RHDV infectivity. [VP2] PMID: 19008396
The precursor of the RNA-dependent RNA polymerase was more active than the mature polymerase. PMID: 19439553
VP2 down regulates VP60 at the transcriptional level. [VP2,VP60] PMID: 19726612

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Subcellular Location

[Capsid protein VP60]: Virion. Host cytoplasm.

Database Links

KEGG: vg:1491968

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ORF1 Antibody

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