Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) POLH Polyclonal antibody

Uniprot No.

Q8H2D5

Target Names

POLH

Alternative Names

POLH antibody; RAD30 antibody; At5g44740 antibody; K23L20.8 antibody; T19K24.15 antibody; DNA polymerase eta antibody; EC 2.7.7.7 antibody; Radiation-sensitive protein 30 antibody; AtRAD30 antibody; Y-family DNA polymerase H antibody; AtPOLH antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana POLH protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Error-free DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis (TLS), where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers and confers resistance to ultraviolet light. Depending on the context, it inserts the correct base, but may cause base transitions and transversions. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci. Exhibits cyclobutane dimer nonmutagenic bypass activity in vitro.

Gene References into Functions

The mutation frequency in AtPolzeta-, AtRev1- or AtPoleta-knockout plants rev3-1, rev1-1 and polh-1, respectively, were analyzed. PMID: 21455019
Arabidopsis thaliana disruptants of AtREV3, AtREV1, and/or AtPOLH genes that encode Translesion synthesis-type polymerases. PMID: 21030509
Transgenic plants over-expressing POLH showed increased resistance to ultraviolet light.[POLH] PMID: 18339443
Evolutionary conservation of efficient T[CPD]T bypass by HsPoleta and AtPoleta may reflect a high degree of exposure of human skin and plants to solar UV-B radiation PMID: 18366182
AtPolH confers UV resistance by interacting with PNCA2 and catalyzing translesion synthesis. PCNA1 does not interact with AtPolH to restore UV resistance in deficient mutants. PMID: 18494853
Results showed that crossing two lines homozygous for insertions in AtREV3 and AtPOLH and selfing F1 plants yielded non-Mendelian F2 genotype distributions: frequencies of +/++/+ and 1/1 2/2 progeny were only 0.42 and 0.25%. PMID: 19082841
AtPolH is a Y-family DNA polymerase, homologous to Polymerase eta from yeast, human and other organisms. It is involved in DNA translesion synthesis. [AtPolH] PMID: 16857217

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Subcellular Location

Nucleus.

Protein Families

DNA polymerase type-Y family

Tissue Specificity

Constitutively expressed in roots, stems, leaves, flowers and siliques.

Database Links

KEGG: ath:AT5G44740

STRING: 3702.AT5G44740.2

UniGene: At.19023

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POLH Antibody

Product Details

Customer Reviews and Q&A

Target Background